FastQCFastQC Report
Fri 30 Jun 2023
SRR2052934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8749582
Total Bases446.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8060799.212771535828796No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3329883.805758949398954No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA753860.8615954453595611No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC708010.8091929420171158No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG624130.7133255051498459No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG508600.5812849116677802No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT366490.4188657240997341No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT307230.3511367743053325No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC274260.31345497419191No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA211940.24222871446887406No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC209880.2398743162816235No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA202380.2313024782212453No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA164670.18820327645366372No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA159450.1822372771636405No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT139940.15993906908924335No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT135320.15465881684405036No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC131510.15030432310937825No Hit
AGTGGCTTTAACATATCTGAACACACAATAGCAGATCGGAAGAGCACACG125040.14290968414262534No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA123500.14114960006089433No Hit
AAGTGGCTTTAACATATCTGAACACACAATAGCAGATCGGAAGAGCACAC120830.1380980257113997No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCAGATCGGAAGAGCACA95280.10889663071904462No Hit
TCGATGTTGGATCAGGACATCCCGATGGTGCAGATCGGAAGAGCACACGT91600.1046907155107524No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC90620.10357066200419633No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT89080.10181057792246531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT88320.10094196499901367Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)