FastQCFastQC Report
Fri 30 Jun 2023
SRR2052936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16165711
Total Bases824.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4828862.987100289000589No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4741072.932793985986759No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC4339002.6840761906482182No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG3507802.1699014661340907No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA3041341.881352450257214No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2936871.8167280115300837TruSeq Adapter, Index 3 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1703371.053693215225733No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1594260.986198503734231No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1593300.9856046541967749No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1460120.9032204027400961No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC946120.5852634628937756No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT895630.5540306887831905No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT830610.5138097544858992No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG822850.5090094707247952No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT801150.49558599680521326No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC691660.4278562198718015No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT584570.3616110667820302No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA561130.3471112405758089No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA552120.3415377152294755No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG517950.32040038325564524No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG497720.307886241440293No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA490870.30364887755323594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC444750.27511935602461285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA444620.275038938899749No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC420560.26015558486725393No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT402030.2486930516078136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT399150.24691150299544512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT388510.24032967062197264No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG386540.23911104188365115No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC375650.2323745611931328TruSeq Adapter, Index 3 (100% over 50bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA347960.21524571359713163No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA346700.2144662860792204No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC332630.2057626787958785No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT329970.2041172207025104No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG281070.17386800988833712No Hit
TCGCGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCT272410.16851099218586799Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA265470.16421795490467447No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG263450.16296839650294379No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC261060.16148995859198523No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTC236010.14599419722398846No Hit
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA216330.13382028170613713Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC212010.1311479587875844No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGA207880.12859316858998654No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG205360.1270343135541641No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGG205350.12702812762148227No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA205320.12700956982343678TruSeq Adapter, Index 3 (100% over 40bp)
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG200730.1241702267224745No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA198510.12279694966710712No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT184110.11388920660526469No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGAGATCGGAAGAGCA180940.11192826594512298No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCA178690.1105364310917101No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAGATCGGAAGAGCACACGTC178460.1103941546400279No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC174650.10803731428824874No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT170510.10547633815796906Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA168050.10395459871823765No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA167210.10343498037296349Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC164890.10199984399077776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)