FastQCFastQC Report
Fri 30 Jun 2023
SRR2052937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17650994
Total Bases900.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC13772197.802501094272651No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG12764007.231320797004407No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC4122152.335364229345951No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG4077542.31009086513768No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT2968601.6818316294255156No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2218351.2567847453803451No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG2188851.2400718055878326No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2160031.2237441132210458No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG2122461.2024591929497002No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1547510.8767268290952905No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1415980.8022097792339626No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1168310.6618947352200109No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1141720.6468304277934716No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1051680.5958191363047316No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT655310.3712595449298776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA583000.3302930135266037No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAGATCGGAAGAG571570.32381745753242No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT530140.300345691579749No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG473390.26819452774161046No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG469250.2658490507673392No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC423320.23982785332089512No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA412520.2337092177358397No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA407270.2307348809931044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA404290.22904659080389467No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG402420.22798716038314898No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA399610.22639518205037062No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG391990.2220781447209149No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT373400.21154615994997222No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAAGATCGGAAGA365710.20718946479727998No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC355380.20133710316824083No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG351620.1992069115201104No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA345750.1958813197715664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT327500.1855419587134866No Hit
ATCTGAAGGTCCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA320360.18149686074336666Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC315500.1787434747300917No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA301990.1710895148454529No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC290830.16476692474089563No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC289500.16401342609940267No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC282130.1598380238529343No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA278270.15765117817160892No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC276000.15636513161808338No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA275950.15633680460148588No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG272090.15414995892016053No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG267860.15175349331601382TruSeq Adapter, Index 6 (100% over 49bp)
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACAGATCGGAAGAGC267520.15156086960315096No Hit
TAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG262560.14875082955668106Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC259470.14700021993095685No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC253540.14364063576249586No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG241830.13700644847536633No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC240390.13619063039735893No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA237280.13442868996499574TruSeq Adapter, Index 6 (100% over 38bp)
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG237130.1343437089152033No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA233640.13236648315669927No Hit
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC228070.12921085350774014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC216250.12251434678409612No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG214520.12153423200982336No Hit
GTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC201550.11418620390443734Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA198310.11235061322892069No Hit
AAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCT191660.10858312002145602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG189920.10759733984386376Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC186880.10587505723473703No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG183530.10397714712270595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT180990.10253813467955403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG180630.10233418016005218No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC179740.10182995926461705No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG178630.10120109949615302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)