FastQCFastQC Report
Fri 30 Jun 2023
SRR2052938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18658813
Total Bases951.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC10884435.833398941293854No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8793384.712722079373431No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4762792.5525685905100177No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA4409322.3631299590172214No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG3372001.8071889138928612No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2879281.5431206690371997No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1908811.0230071977247428No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1479760.7930622382034699No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1007770.5401040248380216No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA911970.4887609946034616No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG801050.42931455500411525No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT750150.4020352205684252No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT695280.37262820523470597No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG685130.36718841654075207No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG560500.30039424265627185No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG548760.29410230972356066No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC489350.26226212782131425No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT456610.24471545965973293No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT417080.2235297604408169No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC414470.2221309576338002No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT413600.22166469003146128Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT392270.21023309467756604No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA390860.20947741959791333No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG389710.20886108885918947No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA385180.20643328168839037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA383980.20579015396102635No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC372830.1998144254942691No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA345570.18520470728765007No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC330120.17692443779783848No Hit
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT297380.15937776963625713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT289920.15537965893114422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA284100.1522604894534288No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC280900.1505454821804581No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT256050.1372273788262951No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT254780.13654673531483486No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA247190.13247895243925753No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA243960.13074786697310273No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC243890.1307103511890065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG243390.13044238130260483TruSeq Adapter, Index 5 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG238890.1280306523249898No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA207660.1112932532203415Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG203240.10892439942455075No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC202950.10876897689043778No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGG201200.10783108228803193No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC197000.1055801352422579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)