FastQCFastQC Report
Fri 30 Jun 2023
SRR2052940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15616355
Total Bases796.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC12825838.213075330318759No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC11998467.683265397078896No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8440015.40459665523741No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA8399185.378450989363396No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5214893.3393772106230935No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3224842.0650401454116536No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG2322761.4873893427755709No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1666661.0672528896788014No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG1114650.7137709151719464No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1075160.6884833240535323No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA987810.6325483763656756No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG955770.6120314247466838No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC768110.4918625377048613No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA750350.48048984542167494No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA710160.4547540062966038No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT699610.44799826848198576No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC650200.4163583627549451No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG503400.32235435221599407No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC484520.3102644631221562No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG455540.29170699564655134No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA445220.28509853931983486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT443460.2839715157602398No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT442070.2830814232898778No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG410720.26300631613459097TruSeq Adapter, Index 7 (100% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT371650.23798767382017125No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG364910.23367168587035836No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC363760.2329352784308502No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA334340.21409605506534654No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG331630.21236069492528825No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC327760.2098825238027696No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC315510.20203818368626994No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG302550.19373919202016093No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT294710.18871881434560114No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC289540.18540818263929065No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA280800.1798114860990289No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG277060.1774165610348894No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA275220.17623830913167637No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC262310.16797133518032858No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT245930.15748233182455187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT238190.15252598957951455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT238040.15242993643523087No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT228540.1463465706305985Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCAGATCGGAAGAGCAC214760.13752248844240542No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG203290.13017762467618085No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA202270.12952446329505188No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGAGATCGGAAGAGCA198410.12705269571548547No Hit
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC197390.12639953433435652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC196840.12604733947198307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG193410.12385092423936314No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC186950.11971423549221313No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC177820.11386780077681379No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA177560.11370130866005543No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA176870.11325946419635055No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA172970.11076208244497517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAGATCGGAAGAGCACACGTC162870.1042945040632081No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT162690.1041792402900677No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAGATCGGAAGAGC161260.10326353364789671No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGC157710.10099027589984987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)