FastQCFastQC Report
Fri 30 Jun 2023
SRR2052941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12189412
Total Bases621.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG126669710.391780998131821No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7695476.313241360616903No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2656092.1790140492420798No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG2588542.123597102140776No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1830101.5013849724662682No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1700861.3953585291891029No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1266141.03872114586003No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1200840.9851500630219079No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1094920.8982549773524761No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG972370.797716903817838No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT889700.7298957488679519No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA750230.6154767760741864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA717840.5889045345255375No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT709150.5817753965490706No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT697790.5724558329802948No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG677180.5555477163295489No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG599410.49174644355281455No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT567920.4659125477094383No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT513590.4213410786344739Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG459860.3772618400296913No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT456990.37490733761398826No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG436360.35798281328090314No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA356920.29281149902882925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT343850.2820890786200352No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC327210.26843788691365916No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAAGATCGGAAGA306120.25113598588676794No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC296610.24333413293438602No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC279570.22935478758122213No Hit
ATCTGAAGGTCCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA274180.22493291719075537Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC260070.21335729730031275No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA256810.21068284507899152No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC253480.20795096596948237No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG246410.20215085026250654TruSeq Adapter, Index 10 (100% over 49bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG242140.19864781008304583No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG231590.18999275764901538Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG230240.18888523909110627No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC221160.1814361513090213No Hit
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC215270.1766040888600697Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG210040.1723134799283181No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG209670.17200993780503931No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA208940.17141105739965143Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC207070.16987693910091806No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA204060.16740758290883925No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC202160.1658488530865968No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT199110.1633466815298392No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT198240.1626329473480755No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG194550.1596057299564573No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC193270.15855563828673605Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG192030.1575383619816936No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC189660.1555940516244754No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC187860.1541173602139299No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAGATCGGAAGAG184960.15173824627471777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC178280.1462580803733601No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG171210.14045796466638422No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA170690.14003136492555998No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA167610.13750458184529327No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA166000.13618376341697205No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG162200.13306630377248713No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG157950.12957967127536588No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT155990.12797171840610522Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT155450.12752871098294158No Hit
GCCCGACTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG152500.12510857783788093No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC148160.12154811077023239No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG141150.11579721811027471No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA139180.1141810613998444No Hit
ATCTGAAGGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA136840.11226136256613528TruSeq Adapter, Index 10 (100% over 35bp)
TCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGAGATCGGAAGAGCA136060.11162146295489889No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA134730.11053035207932917No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT133070.10916851444515946No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC132670.10884036079837156No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA132620.1087993415925231No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA131860.10817584966362612No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC129410.1061659085770503No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG129280.10605925864184423Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC128200.10517324379551696No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC126860.10407392907877755No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA126680.10392625993772299No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC124250.1019327265334866No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG123220.1010877308930078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)