FastQCFastQC Report
Fri 30 Jun 2023
SRR2052942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15349821
Total Bases782.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC8241585.369170103025957No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC5861593.8186699375842883No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA5203783.390124223598438No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA4720843.075501662201794No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG3913222.5493587189062334No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3487242.271844082090599No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA2806821.8285685546430803No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2391191.5577966674660244No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2322031.512740767465627No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1979891.2898456600894563No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1770891.153687720527816No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1379120.8984599885562183No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1194210.7779960430808932No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1188790.7744650572798211No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA1121590.730686045133686No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1104160.719330863858282No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT848280.5526318515375521No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGG723900.47160159066350027No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG716290.46664387812730845No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG692620.45122350286690643No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT692600.4512104733990057No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT685870.44682605745044196No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA639300.41648694144381226No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC611580.3984280989335316No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC588020.38307938574658296No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC547550.35671425744964713No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG544550.3547598372645518TruSeq Adapter, Index 9 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT510830.33279215438408044No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC474480.3091110964746755No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT468240.3050459024896772Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC441720.2877688280534346No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT439660.2864267928596692No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA433370.2823290252049193No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG391690.25517561409999506No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA378250.24641981167076804No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGAACCTGAGATCGGAAGAGCA368200.2398725040506987No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGC329210.2144715563784099No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTC312600.20365058328693214No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC307740.20048442258707774No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGGA305460.1989990632464053No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC304480.19836061931927415No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG293360.19111623516652082No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG293160.19098594048751447No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA293140.19097291101961383No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA289900.1888621372197109No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGA287590.1873572336771875No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA286270.18649728879574556No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT284990.1856634028501049No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCG274000.1785037102387057No Hit
TGCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGT267120.1740215732808871No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA262190.17080980944338048No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC259510.16906386074469534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGT257220.16757198667007256No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG256260.16694657221084208No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCAGATCGGAAGAGCAC235530.15344152873183342No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG229830.1497281303801523No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA228060.1485750224709461Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC227710.14834700678268495Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCAGATCGGA211560.13782571145292183No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT207380.13510255266168902No Hit
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG202270.13177352361307668No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC201660.13137612484210728No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC198300.12918717423480053No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA194410.12665294272812694No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT192650.12550634955287102Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG188820.12301120644989932No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGA187100.12189067221044467No Hit
AGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCA186220.1213173756228167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC180490.11758443306928465No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA176870.11522609937926964No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT176600.11505020156261105No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG175640.11442478710338055No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA175210.11414465354351688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC173530.11305017823986352No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG172480.11236613117508015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGGAAGAGCACA170380.11099803704551342No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT167390.10905013159436842No Hit
TGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGA163510.10652241482164515No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG163290.10637909067473816No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG163020.10620319285807958Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC159980.104222713737183No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG159810.10411196326002758No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG158720.10340185725944295No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC156610.10202724839592592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)