FastQCFastQC Report
Fri 30 Jun 2023
SRR2052943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076928
Total Bases615.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6001224.96916103167958No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3754713.1089942740405507No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2549252.110843088573518No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2378481.9694412353870123No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2028091.6793095065235135No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1655881.3711102691015462No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1470191.2173542808237328No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG1254031.0383683665249972No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1185680.9817728481945077No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1032410.8548614349609437No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT966260.8000875719388241No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT864400.715744931161302No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA852700.7060570370213353No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT816390.6759914441818317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT648860.5372723924494706No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG482930.3998781809413785No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA452080.3743336053671927No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACAGATCGGAAGAGC398700.3301336233850198No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC370130.30647694513041723No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC364910.3021546538987398No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAGATCGGAAGAG359120.29736038833716655No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA340930.28229861103750886No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG332890.2756412889105574No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC329500.27283428368538754No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA321970.26659925437992177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC313890.2599088112473636No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG308270.25525530995961887TruSeq Adapter, Index 12 (100% over 49bp)
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAAGATCGGAAGA304420.2520674131699717No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG300070.2484655038102405No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC286150.23693939385910057No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT285110.23607824771332578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG282490.23390882184608536No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC279790.23167315396763152No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG268050.22195213882205805No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC262680.2175056438193554No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT255110.21123749350828291No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCT252190.20881966009899205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG240960.19952093777490434No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC240140.1988419571599665No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA237380.19655660777310258No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT225980.18711712117518628No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA224310.18573431919110553No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT215270.17824897192398598No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC207570.17187317834469162No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG201240.16663177920742758No Hit
AGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGA197630.16364260845142076No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG195980.1622763669701434No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG190580.15780503121323566No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA190300.15757318417398863No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC189880.15722541361511802No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGC187650.15537891755254318No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC183920.15229038377971615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA182810.15137127587412957No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC181590.15036108520312452No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT172740.14303306271263685No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG172710.14300822195843182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC171820.14227127958368221No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC166370.13775854256976608No Hit
ATTTGGTGTATGTGCTTGGCTGAGGAGCCAGATCGGAAGAGCACACGTCT165070.13668210988754756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA164250.13600312927260974No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGGAAG162920.13490185583618616No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC161670.13386682441097603No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT160050.13252542368390371Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT159720.13225217538764825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC159160.13178848130915413No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG157440.130364278068065No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGATCGGAAGAGCACA153550.1271432602728111No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG147520.12215026867759748No Hit
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC145080.12012988733558733Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG141780.11739740437303262No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC137910.11419294708058209No Hit
TAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG133810.1107980440058929Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT131560.10893498744051466No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAGATCGGAAGAGCACACGTCT127700.10573881039946582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC123630.10236874807898168No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAGATCGGAAGA123370.10215346154253796No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC122200.10118467212854129No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA121040.10022416296594631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)