FastQCFastQC Report
Fri 30 Jun 2023
SRR2052944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12808901
Total Bases653.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT9060157.073323464675073No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA4443893.469376490613832No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG3611872.819812566277154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA2913582.2746526029048084No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC2394751.8695983363443907No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC1944941.5184284740743956No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC1592811.2435180816839788No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC1294561.0106721880354919No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT1132910.8844708847386673No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA1027260.8019891792434027No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG924520.721779331419612No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC913150.7129026916516882No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGC837820.6540920255375539No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA829390.6475106646542119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG815280.636494887422426No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT784260.6122773530687762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT777890.6073042488188487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGGAAG744550.5812754739848486No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC732220.5716493553974693No Hit
AGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGA678270.5295302071582878No Hit
ATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG669700.5228395472804419Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGG582690.45491022219626803No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT558900.436337200201641Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC512870.4004012522229659No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGATCGGAAGAGCACA506680.3955686752516863No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAGATCGG474980.37082026006758895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC471250.3679082225711636No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCT458610.35804008478166865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC447820.3496162551338323No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC447700.34952257028140044No Hit
AGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC440740.3440888488403494TruSeq Adapter, Index 11 (100% over 36bp)
TCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAA434150.33894398902763007No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC422210.3296223462106546No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA417520.325960829894774No Hit
AAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCAGATCGGAAGAG400420.3126107384232262No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG382300.2984643257060071No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA368980.2880653070860646No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAGAT347100.2709834356593122No Hit
AAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCA344080.2686257002064424No Hit
AATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGA341450.2665724405239763No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGC327020.25530683701903856No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAGATCGGAAGAGCACACG325780.25433876021057544No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCAGATCGGAAGAGCAC319940.24977943072555564No Hit
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAGATCGGAAGAGCACACG306560.23933356967939717No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACG306290.23912277876142535No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT301160.23511775131996102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC297430.2322057138235357No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA297280.2320886077579958No Hit
ATATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGT297190.23201834411867187No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAGATCGGAAGAGCACAC291970.22794305303788356No Hit
AAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGA270910.21150136143608264No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG268700.20977599873712818No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAGATCGGAAGAGCACACGTCT267680.20897967749145693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT265250.20708255922971064No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC263940.2060598329239956No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCAGATCGGAAGAGCACACG246350.19232719497168416No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC241800.18877497765030737No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA238550.1862376795636097No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC237240.18521495325789464No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCAGATCGGA235270.18367696026380406No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC227280.17743911050604577No Hit
AGCTAAAAGAGCACACCCGTCTATGTAGCAGATCGGAAGAGCACACGTCT225420.17598699529335107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA224150.17499549727178No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC223280.17431628209164862No Hit
AAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAGATCGGAA223270.1743084750206126No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGA223240.17428505380750464No Hit
AGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAG222510.17371513762187715No Hit
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCAGATCGGAAGAGC216810.16926510713136123No Hit
AGGACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGT213560.16672780904466356No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGA212460.16586903123070432No Hit
AATAGAAGCCGGCGTAAAGAGTGTTTTAGATCAGATCGGAAGAGCACACG209040.16319901293639477No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCAGATCGGAA208750.16297260787635098No Hit
ACCGCACGTTCGTGGGGAACCTGGCGCTAAACCAGATCGGAAGAGCACAC205940.160778820915237No Hit
ATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGATCGGAAGAGCACAC204750.15984977946195383No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCAGATCGGAAGAGCACACGTC203030.15850696324376307No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA199870.15603992879638934No Hit
ATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAGATC198540.15500158834860228No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCG197630.15429114488432694No Hit
AGAAGCCGGCGTAAAGAGTGTTTTAGATCAGATCGGAAGAGCACACGTCT197200.15395544082977924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAGATCGGAAGA191940.149848921464847No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC179050.13978560689945219No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACA172760.13487495921781267No Hit
AGCTTAAAACTCAAAGGACCTGGCGGTGCTTCAGATCGGAAGAGCACACG170180.13286073489052652No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGG170060.1327670500380946No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG169100.13201757121863927TruSeq Adapter, Index 11 (100% over 49bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC168690.1316974813061636No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA168570.13160379645373166No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGG168520.1315647610985517No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG167620.13086212470531233No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC156880.122477330412656No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC156060.1218371505877046No Hit
ATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACAGATCGGAAGAGCA154720.12079100306888156No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACAC153970.12020547274118208No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACAGATCGGAAGAGC153040.11947941513483476No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA147840.11541973819611848No Hit
AGACGAGCTACCTAAGAACAGCTAAAAGAGCAGATCGGAAGAGCACACGT146720.11454534624008726No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG145760.11379586742063195No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA145210.11336647851365235No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG145170.11333525022950838No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG145020.11321814416396848No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGATCGGAAGAGCACACGT144080.11248427948658515No Hit
ATTTGGTGTATGTGCTTGGCTGAGGAGCCAGATCGGAAGAGCACACGTCT143000.1116411158146979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTGGACCAATCTATCACCCTATAGAAGAACTAGATCGGAAGAGCACAC141520.11048566930137098No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACAGATCGGAAGAGCACACGTC140000.10929899450390006No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAA138490.10812012677746513No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACACGTCT138000.1077375802967015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCAGATCGGAAGAGCACACG136960.10692564490895823No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT136900.10687880248274227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGATCGG136810.10680853884341833No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT136460.10653529135715858No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT133860.10450545288780044No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT133260.10403702862564086No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTAGATCGGAA132940.1037872023524891No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG132750.10363886800280524No Hit
AGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCAGATCGGAAGA132430.10338904172965345No Hit
ATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAGATCGGAAGAGCACA131000.10227263057150648No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC130070.10154657296515915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)