FastQCFastQC Report
Fri 30 Jun 2023
SRR2052947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910736
Total Bases148.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1295734.451554520918421No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC865242.9725815051588325No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT606512.08369979276719No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC495711.7030400558484176No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG360931.2399956574557087No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG276140.9486947631114604No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC224950.7728285904321106No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT216600.7441416878755064No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT172960.5942139719988346No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG150250.5161924681592559No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT150030.5154366455769263No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT142930.4910441895108316No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG142360.48908592191115924TruSeq Adapter, Index 4 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA139720.48001605092320293No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT112890.38784005145090455No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC104120.35771021487348903No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT99890.3431778079496045Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC97120.33366131452663517No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG95980.3297447793272904No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG85660.29428982910164303No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG80690.2772151098553768No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC78960.2712715959125115No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA77870.2675268385727871No Hit
ATCTGAAGGTCCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA76300.262133013780707Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA65230.22410139566075385No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT63080.2167149476970773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA62970.21633703640591245TruSeq Adapter, Index 4 (100% over 40bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT59190.20335063021861138No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC55100.1892992013016639TruSeq Adapter, Index 4 (100% over 38bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC54370.1867912445512063No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT52370.17992013016639088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTGAAGGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG51400.17658763968975547TruSeq Adapter, Index 4 (100% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC51110.1755913281039572TruSeq Adapter, Index 4 (100% over 50bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC50420.17322079364119589No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA49910.171468659473068No Hit
TTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT49710.17078154803458642TruSeq Adapter, Index 4 (100% over 41bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTAT49150.16885763600683815TruSeq Adapter, Index 4 (100% over 48bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA49050.16851408028759737Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC48740.16744905755795098No Hit
GTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC47320.16257056634473205Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC45580.15659269682994265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG45030.15470314037411842No Hit
TAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG44450.152710517202522Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT44260.15205776133596452No Hit
GTAGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA43670.15003078259244398TruSeq Adapter, Index 4 (100% over 40bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG43130.1481755817085438No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC40240.1382468214224856No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT39290.13498304208969827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC39170.13457077522660937No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC39010.13402108607582414No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC38990.13395237493197598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA38680.13288735220232958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG38600.13261250762693697No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG38580.1325437964830888No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT38530.13237201862346842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC36280.12464201494055112No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG36230.12447023708093075Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGA36200.12436717036515849No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT35880.12326779206358804No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG34990.12021014616234521No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC32910.11306418720213719No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG32490.11162125318132596No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCT31190.10715502883119594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA31040.1066396952523348No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT30850.10598693938577734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)