FastQCFastQC Report
Fri 30 Jun 2023
SRR2052948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339594
Total Bases119.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC741373.168797663184296No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC588472.5152654691369527No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG324881.388616999359718TruSeq Adapter, Index 3 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA278191.189052459529303No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA233310.9972243047297951No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG191550.8187317970553866No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC188770.806849393527253No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC184330.7878717418492269No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC176930.7562423223858498TruSeq Adapter, Index 3 (100% over 50bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT147910.6322037071389309No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG126750.5417606644571665No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT113980.48717854465347404No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA97180.4153712139798615No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA91280.39015316332662847No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT88180.37690300111899755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC82580.35296722422779336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT77610.3317242222368496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT77570.33155325240191247No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTAT76620.3274927188221546TruSeq Adapter, Index 3 (100% over 48bp)
GCGCGACCGCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT76590.3273644914459517Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG68540.2929568121648457TruSeq Adapter, Index 3 (100% over 38bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT65560.2802195594620263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG65280.2790227706174661No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT63810.272739629183525TruSeq Adapter, Index 3 (100% over 35bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG63800.2726968867247907No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA62860.2686790956037671No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT59510.25436037192777894No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA58990.25213776407359567No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC55710.23811823760874748No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG55030.23521175041481554No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT51130.21854219150844123Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG47190.2017016627671297No Hit
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA46230.19759838672863753TruSeq Adapter, Index 3 (100% over 36bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT44040.18823778826582732Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC40900.17481665622325926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT40620.17361986737869903No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA40280.17216662378173306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG36980.1580616123994163Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG34820.14882924131280897No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC34260.14643566362368857No Hit
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG34040.1454953295315341No Hit
GCGCGACCGCTCTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC30870.13194597011276316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT30270.12938142258870558TruSeq Adapter, Index 3 (100% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC29580.12643219293603933No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC29190.12476523704540189No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT28580.12215794706261002No Hit
CTGATGTTCGCCGACCGTTGACTAGATCGGAAGAGCACACGTCTGAACTC28530.12194423476893854Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC28450.12160229509906419No Hit
GTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC28380.12130309788792415Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC27530.11766998889550923No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT27190.11621674529854326No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC26230.1121134692600511No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA26080.11147233237903671Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA24920.10651420716585869Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC24170.1033085227607867Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)