FastQCFastQC Report
Fri 30 Jun 2023
SRR2052949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846689
Total Bases196.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1382063.592856090003637No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1013102.633693547879748No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC749191.9476230077347039No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG595171.5472267188743358No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG531831.3825656298182671No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT422261.097723262785216No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC251890.6548228879433715No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT245570.6383931739737733No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT213590.555256741576977No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT208760.5427004886540087No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT208310.5415306514251608No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT193050.5018601711757826No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT188300.4895118893157206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA172710.448983528431854No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG155640.40460770288422065TruSeq Adapter, Index 6 (100% over 49bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG144870.3766095985404591No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG134210.34889745440819364No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG132630.34479002591579405No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT115300.2997382944137153Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGAAGGTCCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA95640.24862940570449027Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG90960.2364630985244713Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA84770.22037133753209578TruSeq Adapter, Index 6 (100% over 38bp)
GTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC80900.21031073736400316Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG79150.20576137036292771No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA77320.20100403229894592Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA74130.1927111861655569TruSeq Adapter, Index 6 (100% over 40bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC73610.19135937425666594Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC68730.17867313941938118No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC68020.1768273962360877TruSeq Adapter, Index 6 (100% over 50bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT66860.17381181582394625No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG66530.17295393518945776Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT66280.17230402561787553No Hit
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC61550.1600077365235401Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC61540.15998174014067681No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC61350.15948780886627434No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG59090.15361262633917117No Hit
TTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT57620.14979115805826776TruSeq Adapter, Index 6 (100% over 41bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC56460.1467755776461263No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC51670.13432331025461117No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA50690.1317756647340089No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT50640.13164568281969247No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC50220.13055383473943435No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG49070.1275642507101562No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT48870.1270443230528904TruSeq Adapter, Index 6 (100% over 48bp)
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG48590.12631642433271834No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG47880.12447068114942487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC47190.12267693073185797No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA46780.12161107903446314No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA46220.12015528159411899No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG45250.11763363245638002No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT45220.11755564330779016No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT45220.11755564330779016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGGCTGTAGTGCGCTATGCCGAGATCGGAAGAGCACACGTCTGAAC44750.1163338133132156Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTAGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA44480.11563191097590682TruSeq Adapter, Index 6 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG43330.11264232694662864No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC43180.11225238120367931No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA42090.10941877547158088No Hit
GTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC39710.10323163635011824Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC39680.10315364720152838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)