FastQCFastQC Report
Fri 30 Jun 2023
SRR2052951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087976
Total Bases106.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1320656.325024808714276No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG822123.9374015793284975No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT326411.5632842523094135No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC324371.5535140250654222No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA260281.246566052483362No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC228951.096516435054809No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG205980.986505592018299No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG193150.9250585255769224No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT165910.794597255907156No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT156110.7476618505193546Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT119430.5719893332107265No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG102850.4925822902178952No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT98370.4711261048977574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT95170.4558002582405162No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA92020.4407138779372943No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC75830.36317467250581426No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC75740.3627436330685793No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC71330.3416227006440687No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT70980.33994643616593295No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG66810.3199749422407154No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG64020.30661271968643317TruSeq Adapter, Index 8 (100% over 49bp)
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG61710.2955493741307371TruSeq Adapter, Index 8 (100% over 38bp)
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT60710.29076004705034925Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGAAGGTCCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA59840.28659333249041175Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA52670.25225385732403055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG50160.2402326463522569No Hit
AGTGACGAAAAATAACAATACAGGACTCAGATCGGAAGAGCACACGTCTG48980.2345812403973992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG48640.2329528691900673No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC47740.2286424748177182Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG46620.22327842848768376No Hit
ATTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA45580.21829752832408034TruSeq Adapter, Index 8 (100% over 40bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGATCGG43920.21034724537063645No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG41820.20028965850182184Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC41490.19870918056529388No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA39030.18692743594753963No Hit
ATCTGAAGGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC38710.1853948512818155TruSeq Adapter, Index 5 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA37540.17979133859776167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC35300.16906324593769276No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT35270.16891956612528114No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA33770.16173557550469927No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG33460.16025088410977906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG33400.15996352448495577Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC33150.15876619271485878Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCT31000.1484691394920248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT30770.14736759426353557No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT30280.14502082399414554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGT29970.14353613259922526No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT29720.1423388008291283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT29700.14224301428752056TruSeq Adapter, Index 8 (100% over 41bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA28610.13702264776989773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAGATCGGAAGAGCACACGTCTGA28420.13611267562462404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG27930.13376590535523397No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG27830.1332869726471952No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA26640.1275876734215336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG26610.12744399360912192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC26610.12744399360912192Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT26150.1252409031521435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT25420.12174469438346035No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG24780.1186795250520121No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC24770.11863163178120822Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG24680.1182005923439733No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC24630.11796112598995391No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG24390.11681168749066081No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC24350.11662011440744531Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT24330.11652432786583752No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC23920.1145607037628785No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA23610.11307601236795826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC23310.11163921424384188Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA22910.10972348341168672No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC22760.10900508434962854TruSeq Adapter, Index 8 (100% over 50bp)
TCCGCGCGGCGCCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC22700.10871772472480527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT22680.10862193818319751No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA22340.10699356697586562No Hit
TCTCGTCGCGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC21930.1050299428729066Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCG21840.10459890343567167No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT21710.10397629091522126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT21390.10244370624949713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)