FastQCFastQC Report
Fri 30 Jun 2023
SRR2052952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291662
Total Bases116.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1560646.810079322343348No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA953684.161521201643175No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC862493.763600391331706No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC838163.6574329024088192No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA381841.6662143021091245No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA359591.5691231953054159No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG280541.2241770383241506No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC188460.8223725837405342No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG187080.8163507532960794No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG157480.6871868539077752No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA157220.686052306142878No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG150700.6576013391154543No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG117570.5130337719960447No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT109590.4782118829041979No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT100420.4381972559653212No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA94360.4117535657527157No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT92330.4028953658960178No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG86790.37872077121320685TruSeq Adapter, Index 7 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA86750.37854622540322264No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC76070.3319424941374426No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT71500.3120006353467483Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC68660.29960788283787054No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA67680.29533151049325773No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA59480.25954961944649774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT58110.2535714254545391No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC56490.2465023201501792No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT47390.20679314837877488No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG47270.2062695109488223No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT47200.20596405578134996No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC46830.20434950703899615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGG46390.20242950312917No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA45740.19959313371692686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA43910.1916076629101499No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT43490.18977493190531589TruSeq Adapter, Index 7 (100% over 38bp)
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT42710.18637128861062408No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC42490.18541128665571102No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC40510.17677126906149335Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG39200.171054893784511No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT38880.16965852730463743Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC36550.15949123387305808No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG36020.15717850189076749Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG35800.1562184999358544No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG35280.1539494044060599No Hit
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC34090.14875666655903008Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT33650.14683666264920395No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC33640.1467930261967079No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC32450.14160028834967808No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC30670.1338329998053814No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG29740.12977480972324887No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG29580.1290766264833121No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC29040.12672025804852546No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT28560.12462570832871514No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT27790.1212657014865194No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC27150.11847296852677228No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT26930.11751296657185921No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC26920.11746933011936316No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGAGATCG26870.11725114785688291No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCAGATCGGAAGAGCAC26500.1156365991145291No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC26390.11515659813707257TruSeq Adapter, Index 7 (100% over 50bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG26280.11467659715961603No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA26240.11450205134963184No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAGATCGGAAGAGC25260.11022567900501906No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA25170.10983295093255463TruSeq Adapter, Index 7 (100% over 35bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG24710.10782567411773639No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT24660.10760749185525614TruSeq Adapter, Index 7 (100% over 48bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT23840.10402930275058016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC23040.10053838655089625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)