FastQCFastQC Report
Fri 30 Jun 2023
SRR2052953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358678
Total Bases426.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG140368516.793145997489077TruSeq Adapter, Index 10 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3412724.082846593683834No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA2557703.0599336402239685No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC1481111.7719428837909537TruSeq Adapter, Index 10 (100% over 50bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1118341.3379388463103854No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG1089861.3038664726646965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1050101.2562991420413612No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG924041.1054858196475568No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT817510.9780374360634541No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG795280.9514423213814435No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC794250.9502100691042292No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT783520.9373731109153864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG657650.7867870971940779No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC549690.6576279167590856No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG507270.6068782647208087No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA484500.5796371148643362No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC458910.549022225763452No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA368120.44040457115347664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGA266740.31911744895544486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT257110.3075964883442095No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC238690.28555951072645697No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG237130.2836931868891229No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA236130.2824968254549344No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG223680.2676021255992873No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA220740.2640848229827731No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC219360.26243384420359295No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCA207570.24832874289451037No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC201100.24058828441531066No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC200250.2395713771962504No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACAC188830.22590892961781756No Hit
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCAGATCGGAAGAGC187050.22377940626496198No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC180400.21582360272760837No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA180090.21545273068300994No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA175020.20938717821167413No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC174230.2084420526786652Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT170000.20338144381204779No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG166880.1996487961373796No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA154180.18445500592318545No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG153470.1836055893049116No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT152850.18286384521571472No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT150610.18018399560313245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT144380.172730663868138No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA143890.17214444676538562No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG141800.16964405136793162TruSeq Adapter, Index 10 (100% over 49bp)
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG133210.15936730664825227No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT132800.15887679846023497Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACAGATCGGAAGAGCA124860.14937768867277815No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC122290.14630303978691367No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG119930.14347962680222875No Hit
ATATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGT116270.1391009439530988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA113060.13526062374935366No Hit
ACGTTCGTGGGGAACCTGGCGCTAAACCAGATCGGAAGAGCACACGTCTG112980.1351649148346186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA110400.1320783023344122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA110310.13197062980533525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAGATCGGAAGAGCACACG109100.13052303246996713No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGG108440.12973343392340272No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG104960.12557009613242667No Hit
TCCGCGCGGCGCCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC100550.12029414220765533Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG98630.11799712825401337TruSeq Adapter, Index 10 (100% over 36bp)
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGG98150.1174228747656029No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG97890.11711182079271387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGA94370.11290062854437029No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGGAAG94070.11254172011411374No Hit
TCTCGTCGCGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC93720.11212299361214775Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT91660.10965848905771941No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC90790.10861765460997541No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCG90220.10793572859248794No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT89880.10752896570486385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT86840.10389202694493077No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC84990.10167875829168202No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC84800.10145144961918619No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC83690.10012348842723695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)