FastQCFastQC Report
Fri 30 Jun 2023
SRR2052954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257987
Total Bases115.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC803033.5563978003416317No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC798753.5374428639314575No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG697693.08987607103141TruSeq Adapter, Index 9 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA669472.964897494981149No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG631022.794613077931804No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA386341.7109930216604434No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG264391.1709101956742887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG230721.0217950767652781No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT176790.7829540205501626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC142180.6296759015884503No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC140070.6203312950871728No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA121380.537558453613772No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG104930.4647059526914903No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC101770.4507111865568757TruSeq Adapter, Index 9 (100% over 50bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA77630.34380180222472495No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT73240.3243597062339154No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT68490.3033232697973903No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA67360.2983188122872275No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT65390.289594227070395No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA60240.2667863012497415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG56060.2482742371855994No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAGATCGGAAGAG54990.24353550308305583No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC53940.2388853434497187No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA51050.2260863326493908No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC49800.22055042832398947No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT49380.2186903644706546No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC49360.2186017900014482Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT46510.2059799281395331No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA45710.20243694937127626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT44830.19853967272619372Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC44340.1963695982306364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA44270.19605958758841394No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGA43920.19450953437730154No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCG41730.1848106299991984No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC41630.1843677576531663No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT41310.18295056614586355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG39860.176528917128398No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA39520.17502315115188882Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT37950.16807005531918473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC34800.15411957641917334No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT34650.1534552679001252Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGAGATCGG34270.1517723529852032No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGATCGGAAGAGCACACGTCTG29400.13020446973343955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG29300.12976159738740745No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC29070.1287429909915336No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA28970.1283001186455015TruSeq Adapter, Index 9 (100% over 38bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAAGATCGGAAG28150.12466856540803822No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA26590.11775975680993733TruSeq Adapter, Index 9 (100% over 35bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC26410.11696258658707955No Hit
TCGCGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCT26360.1167411504140635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACGACTCTTAGCGGTGGATCAGATCGGAAGAGCACACGTCTGAACT25820.11434963974549012Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAGATCGGAAGAGCACACGTC25090.11111667161945575No Hit
AGGGGGACGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT24820.10992091628516905TruSeq Adapter, Index 9 (100% over 36bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT24770.109699480112153No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT24190.10713082050516677No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG23880.10575791623246725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC23590.10447358642897413No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG23440.10380927790992597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)