FastQCFastQC Report
Fri 30 Jun 2023
SRR2052955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15968518
Total Bases814.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT7101694.447306882204097No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3469612.1727814691382132No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3454882.1635570689778474No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG2221821.3913752046370238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2095591.312325915279051No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA1900141.1899288337214513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC1861951.1660130263810329No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1764961.1052747662619662No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA1529660.9579223319283606No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1521060.9525367350933881No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1443750.9041227244757467No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT1261650.7900858426561564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA1205590.7549792660784175No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1197720.7500508187422277No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC1160250.7265858985786908No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT993390.6220927953364238Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA989020.6193561606656297No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA914090.5724325826604573No Hit
AGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGA869720.5446466603851403No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC865140.5417785169544225No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC832000.5210251821740752No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT820300.5136982655497524No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC803910.5034343199537992No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAGATCGG707470.44304048753929454No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC671260.4203646199353002No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC640020.4008011263161679No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGG567330.3552803084168487No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA557840.34933736493267564No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT553630.34670092741229963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT539100.3376017736899567No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA536380.33589842213284915No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC524780.3286341287275375No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT502860.314907119120259No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG495720.3104358212828517No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG495580.31034814877623584No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG492030.30812502450133444No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGGAAG465080.2912480669777872No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT344260.2155866937683259No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAGATCGGAAGAG336380.21065198411023492No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG334770.20964375028415283No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC334000.2091615514977658No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCT331600.20765859424149444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC323370.20250470331686382No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGA321060.20105810695770265No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC310970.19473942415946177No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG308060.19291708848623274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG300410.1881264122318677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG298230.18676122605742124Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAGATCGGAAGAGCACACG295090.1847948569804662No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG294350.1843314451597825No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGA293470.18378036083248303No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA292720.18331068668989822No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAGATCGGAAGA287670.1801482141298272No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATCGG285720.17892706135910671No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC285510.17879555259918298No Hit
AACACGGGAAACCTCACCCGGCCCGGACACGGACAGATCGGAAGAGCACA278850.1746248462130299No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT276690.17327218468238567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC275880.1727649366083941No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGC271680.17013476140991918No Hit
ATATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGT264760.16580123465433672No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA262330.16427949043236198No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA255950.1602841290594406Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT254420.15932599380856757No Hit
AAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCT253430.15870602394035563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCAGATCGGAA252420.15807352942834144No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACG252230.15795454531221997No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT234710.14698295734143896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT225630.14129676905521227No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA224030.14029479755103136No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGG222360.13924898979354253No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA218140.13660628995126536No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC214950.13460860926480467No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACAGATCGGAAGAGCACACGTC211290.13231659944899082No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCAGATCGGA208930.13083869148032398No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC203240.1272754303185806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGC202030.12651768936854377No Hit
AAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGA190930.11956651205828868No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAG190350.1192032973880231No Hit
AATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGA187950.11770034013175175No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG187360.11733086313958502TruSeq Adapter, Index 12 (100% over 49bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGATCGG187040.11713046883874885No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCAGATCGGAAGAGCACACGTCTGA184780.1157151840890933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGATCGG183560.11495118081715536No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG183200.11472573722871465No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC182630.11436878488035021No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCAGATCGGAAGAGCACACGTCTG177060.11088067158142038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTGGTGTATGTGCTTGGCTGAGGAGCCAGATCGGAAGAGCACACGTCT175290.10977224060492025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC175000.10959063326978746No Hit
GAAGACGATCAGATACCGTCGTAGTTCCGACCAGATCGGAAGAGCACACG172140.10779960920606409No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA171100.10714832772834648No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA170760.10693540878370805No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC170380.10669744055146507No Hit
TTCTGGGCTGTAGTGCGCTATGCAGATCGGAAGAGCACACGTCTGAACTC169140.10592091263572487Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG168400.10545750081504118No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT167390.10482500630302699No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC167150.10467471057739985No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA163950.10267076756903804No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG163290.10225745432356341No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAGATCGGAAGAGCACACGTCTGA162620.10183787875618765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA162250.1016061728458458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)