FastQCFastQC Report
Fri 30 Jun 2023
SRR2052957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543624
Total Bases129.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1503105.909285334624928No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1260874.956982635798373No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG870723.4231474463206824TruSeq Adapter, Index 2 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG784043.082373809965624No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT662222.603450824492928No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC469191.844572940025727No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC335261.3180407167097024No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA314141.2350095768871499No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG270971.0652910964828135No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG177150.6964472736536532No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT176530.6940098064808321No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC131920.5186301119976853TruSeq Adapter, Index 2 (100% over 50bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT103930.4085902633408083No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG78820.30987284284155203No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT78290.3077892015486566No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT74790.2940293062182146No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC69570.273507405182527No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC63650.2502335250807509No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA58740.23093035763147385No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG56650.2227137344198671No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT55800.21937204555390263No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC51510.20250634527744665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC49680.19531188571895847No Hit
TCGAGGCCCTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA45580.17919315118901222TruSeq Adapter, Index 2 (100% over 36bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT41540.1633103005790164No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG40020.15733457460693875No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA39760.15631241095382023Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT37990.14935383531528246No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT37810.14864618355543113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA36220.14239525967674466No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT35810.14078338622375003No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAGATCGGAAGAGCACACG34970.137481011344444No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC34010.13370686862523706No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA32460.12761320069318421No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG30820.1211657068812057No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC30380.11943589146823587No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG29930.11766676206860763TruSeq Adapter, Index 2 (100% over 38bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG28560.11228074589640608No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG28050.11027573257682739No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT27370.10760238148405582No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC26420.1038675527515073No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG26380.10371029680487368No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA25910.10186253943192862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)