FastQCFastQC Report
Thu 29 Jun 2023
SRR2052958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587866
Total Bases131.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2111278.158343592751711No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA545102.1063687223372463No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC449021.7350975668755646No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC329311.2725156557565191No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT266091.028221708542869No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC224590.8678579184548195No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA205540.7942451425228354No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT201650.7792134523194014No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA122760.474367683643589No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC95580.369339061605199No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG94900.3667114139603828TruSeq Adapter, Index 1 (100% over 49bp)
TAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA85400.3300016306872149Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG79430.30693243004081355No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC76050.2938714755709917No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA65870.2545340446530075No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG64260.24831270243513381No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT61310.23691334868188693No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT61300.23687470680475728TruSeq Adapter, Index 1 (100% over 35bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT60860.23517446421105265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG54210.2094776159198351No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC50020.19328666940251157No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC47260.18262151131472804No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG43400.16770574674268296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC43140.16670105793731205No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA42070.16256637708443947No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC41440.1601319388252715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA41180.15912725001990055No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT40910.15808391933740001No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC40100.15495392728989832No Hit
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT39850.15398788036165706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC39510.15267405653924895No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC38170.14749604500387578No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA37920.14652999807563452Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG36330.1403859396120201No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC36250.1400768045949829No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGCACACGT36150.1396903858236864No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA35870.13860841326405618Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT35560.137410515073037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG34830.13458965804257253No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG34630.1338168204999795No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC34520.13339175985155335TruSeq Adapter, Index 1 (100% over 50bp)
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC33590.12979806527849586TruSeq Adapter, Index 1 (100% over 38bp)
GCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC31800.12288116927228845TruSeq Adapter, Index 1 (100% over 36bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG31700.12249475050099194No Hit
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG31410.12137413606423206No Hit
GCGCGACCGCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT31160.12040808913599081TruSeq Adapter, Index 1 (100% over 35bp)
GCGGCGACTCTGGACGCGAGCCGGGCCCTAGATCGGAAGAGCACACGTCT30800.1190169815593234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT30190.11665982705441472No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC29750.11495958446071011No Hit
ATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC29130.11256378807867178TruSeq Adapter, Index 1 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG28420.10982021480246658No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT28120.10866095848857708TruSeq Adapter, Index 1 (100% over 48bp)
GCGGCGACTCTGGACGCGAGCCGGGCCCAGATCGGAAGAGCACACGTCTG26730.10328973756755568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)