FastQCFastQC Report
Sat 1 Jul 2023
SRR2052983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50720722
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC774776115.275336577424902No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA612329712.072574597814281No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC9538921.8806751213044641No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG7857601.5491893037327031No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT7365191.4521066951688897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC5740041.1316952467671892No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT4933240.9726281104594687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4021070.7927864276064525No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT3463430.6828431976973829No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC3372090.6648347789686432No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT3189140.628764708830446No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3028100.5970143721534563No Hit
TCCCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2650880.522642402448451No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC2586170.5098843033030956No Hit
TCACTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2258040.4451908235848851No Hit
TCCCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2091510.41235808906663435No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2043330.40285901292966614No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT1869000.36848844541290243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT1841580.3630823709488994No Hit
TCACTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1802240.35532617221024576No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC1716910.33850267352266794No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC1406300.2772634033088094No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1405090.2770248420359631No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG1368930.2698956059813186No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA1350820.2663250732116944No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1349430.26605102348503634No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG1343240.2648306149900626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1259390.2482989102560488No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA1184550.23354359979339412No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1148800.22649519855020991No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1136500.22407015420640108No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC1127990.22239233897340815No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC1008810.19889503938843772No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC1007200.1985776148848985No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC995130.19619791689873814No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAAAGCAC978960.19300987079797485No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA936160.18457150511382706No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG801940.15810894805480094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG800010.15772843296670738No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT794270.15659674560626324Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC777640.15331800679020302No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAAAGCACA767520.1513227670536709No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA752000.14826287370278365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC737040.14531338887486658No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG722930.14253148841217206No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT707890.13956623093811638No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC672340.13255726131027867No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGAAAGAGCAC643450.1268613644734789No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA626760.12357079617281473No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA623850.12299706616952337No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC616550.12155781220937667No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG615490.12134882464804031No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT601020.11849594727772211No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA567800.11194635596867095No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA558920.11019559224728702Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAACAC556310.10968100966701538No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAACACA551650.10876225302944229No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA542500.10695825662734058No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT539020.10627214652031175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC527040.1039101927610573Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC516550.10184200453613418No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA511370.1008207256986602No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGT507530.1000636386840077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)