FastQCFastQC Report
Fri 30 Jun 2023
SRR2052984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32000428
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC15784374.932549652148403No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC14302944.46960896898004No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT11664413.6450793720633987No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA11159093.4871689841148377No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC9612613.0039004478315103No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC9200862.8752302937948206No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC6048901.8902559678264301No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT5693281.779126204187019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT5397151.686586816901324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT3525461.1016915148759885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG3186990.9959210545558953No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT2800520.8751507948581188No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC2717990.8493605148031146No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT2672470.8351357050599448No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC2233770.6980437886643267No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC2223400.6948032070071063No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA1817010.5678080305675912No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1679470.5248273554341211No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT1400160.4375441478470225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1332830.416503804261618No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC1323210.413497594469674No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1137140.3553514971737253No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACAC1121890.35058593591310716No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT1054840.32963309115740574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA1039920.3249706535175092No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1005530.31422392225503987No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA952300.29758976973682977No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG926170.2894242539506034No Hit
TCATACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC873930.27309947229455805No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC866950.2709182514683866No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACACGTCT759390.23730620102956124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC708710.2214689128532906No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC705960.22060954934727747No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC692470.2163939807305077No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC687040.2146971284259073No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC682130.21316277394789845No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC673350.21041906064506385No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGT660920.2065347375978846No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACAAGATCGGAAGAGCACAC641870.20058169221986658No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC630950.1971692378614436No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG613920.1918474340405697No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCAAGATCGGAAGAGCACAC603300.18852872842825727No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC548150.17129458393493988No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTC544230.17006960031909574No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT523440.16357281221363665No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT516190.16130721751596572No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA488610.15268858279020517No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA472620.14769177462251443No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA470250.1469511595282413No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG464150.14504493502399404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA458960.14342308171628204No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT456570.14267621670560154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCCTGTAATTGGAATGAGTCCACTTCAGATCGGAAGAGCACACGTCT451360.1410481134814822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG449480.14046062133918957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA448370.14011375097858067No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT448260.14007937643834015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC446750.13960750774958383No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG435660.13614192910169826No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG424260.1325794767494985No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA417460.13045450517099336No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC408850.1277639161576214No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT405490.12671393020118354No Hit
TCCCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA395270.12352022291701849No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCAAGATCGGAAGAGCACACGTCT389250.1216389980784007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC369590.1154953302499579No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA356580.111429759626965No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACGCAGATCGGAAGAGCACA356530.11141413483594657No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACACGT349260.10914229022186829No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG348110.10878292002844461No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG347990.1087454205300004No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTC340750.10648295079053317No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCAC339650.10613920538812795No Hit
TCCCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC338430.10575796048727848No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT334720.10459860099371171No Hit
TCACTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA333890.10433922946280594No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT331730.10366423849081019No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGATCGGAAGAGCACACGTCT329810.10306424651570285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACA323800.10118614663528874No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT320970.10030178346364617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC320610.10018928496831356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)