FastQCFastQC Report
Fri 30 Jun 2023
SRR2052985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29304738
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC477464716.293088851365948No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT453806915.485785950381128No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC18490836.309843138676073No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC11555513.9432224236231015No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA6582422.2461965024222366No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT4410111.5049136422922464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT3455451.1791437957916566No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC2052390.7003611497908632No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC1954880.6670866670092734No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1652390.5638644508611542No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA1634270.5576811503996385No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1514120.5166809544586272No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1427050.4869690355191027No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1272860.4343529704991732No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1257920.42925481879414856No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1245550.4250336583797473No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT1214070.4142913681739792No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT1184940.4043509960744232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1149560.3922778630540904No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1000150.3412929335863709No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG947130.323200296143238No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG883720.3015621569454059No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC864520.29501031539677985No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG798040.27232456403466226No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC763880.26066774594606507No Hit
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC728630.2486389743528845No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC718680.245243618967008No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT675120.23037912845356273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC674020.230003762531506No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC672870.2296113345220831No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA664430.22673125417466625No Hit
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC663050.22626034056335873No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC637860.2176644609482603No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT626850.21390738931022008No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT583730.19919304516559747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC568800.19409830587804605No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG543880.1855945615347252No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA541850.18490184078765692No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT524370.17893693504442865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC510090.17406400289263804No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT464410.15847607987486528No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA462490.15782089572000269No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC446710.15243610094722568No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT424440.1448366472343141No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT410490.1400763248591405No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA402740.13743170131737742No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCAC400110.13653423552191457No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT400100.13653082310444134No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC394010.1344526608632365No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA375960.12829324732403338No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC375810.12824206106193475No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA369260.12600692761696078No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA365640.12477163249164692No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT354230.12087806415467697No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC353520.12063578251407672No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT353320.12056753416461187No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC334020.11398156844125343No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAACACAC319500.10902673827010499No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACA317830.10845686455207344No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG312820.10674724339797886No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC305830.10436196358418219No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG303340.10351227163334475No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC299050.10204834453732363No Hit
AGGATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC295880.10096660819830569No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)