FastQCFastQC Report
Fri 30 Jun 2023
SRR2052986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31483076
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC17598045.589682532926579No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT13099444.160787846778377No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC12721144.040628050448438No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT12696304.032738097128756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC11290263.58613624666154No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT4233101.3445636633472537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT3934611.2497539948129592No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA2886680.9168989713711584No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC2812480.893330753322833No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2633110.8363572860542597No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT2225890.7070116020429517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA2212700.7028220495354393No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT1872030.5946147066442936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC1768440.5617113143582285No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1671560.5309392258875848No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1609560.51124610568548No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1499130.4761701175577634No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1435330.4559052616078556No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1314160.4174179168515808No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1117420.3549271996167083No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1103910.3506360051984755No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1067510.339074237854014No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1030230.327232955255071No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC995490.3161984553224723No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA966470.3069808045440033No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA920110.29225543272836496No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC910510.2892061754067487No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT893450.28378739104145984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG871110.2766915151492821No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG829790.2635670034274923No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGT795820.2527770793425649No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC763380.24247313064327006No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG731790.23243916826932667No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC706110.22428240493400325No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC685420.2177106201439783No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACA664630.21110707225685316No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC598350.19005449149886117No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG595510.18915241954121637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT592560.18821540817676138No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC584160.1855473080203472No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT577160.18332389122333537No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC575420.18277121333379243No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA569110.18076696190677174No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC554600.17615813651753723No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC541570.17201940496538523No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG524610.16663238369719655No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG513400.1630717405122676No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC505810.1606609214423648No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA492840.15654124774847286No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG486500.15452746739232215No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG484690.1539525553348091No Hit
AAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACACGTCTGA473160.1502902702391596Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT462980.14705678695436242No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT456430.14497630409430132No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT449420.1427497109875795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA444820.1412886085209717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA431000.13689894850172837No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC429580.13644791252290595No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT428200.1360095817829236No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC425010.13499633898542823No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA418430.13290632719623713No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA393640.12503225542510524No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC392190.12457169051715278No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC376550.11960394213068634No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT373830.11873998588956174No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG363020.11530639509303346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT355380.11287969447458057No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC354420.11257476874241895No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCA353510.1122857245588074No Hit
GAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCAC349250.11093261662234022No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC347990.11053240159887807No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC346030.10990984489571477No Hit
AACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACA344370.10938257748385197No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG338400.10748632058697187No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA330330.10492303865098825No Hit
AACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCACACGTCTGA329430.1046371707770867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG326940.1038462696592925No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTAGATCGGAAGAGCACACGTC324930.10320783140757911No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT322920.1025693931558657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA322860.1025503352976056No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG320610.1018356656128518No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT319860.10159744238460054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGTCTG319530.10149262416416999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA317610.10088277269984675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)