FastQCFastQC Report
Fri 30 Jun 2023
SRR2052987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31451899
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT433349513.778166463017067No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC383350612.188472308142666No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC15727895.000616973874932No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC13326864.237219507795062No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT6196251.970071823008207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA4603501.4636636089922583No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC3341661.0624668481861779No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT2950010.9379433655182474No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1953160.6209990690864167No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT1744920.554790030325355No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC1617460.5142646553710477No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1572830.5000747331663503No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1554590.4942754013040675No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA1497960.4762701291899736No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC1465060.46580971152171124No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT1350520.4293921966365211No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT1344050.4273350871437047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT1332180.42356107019165995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1281810.40754613894696784No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1275690.4056003104931756No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1220180.38795113770395867No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1204950.38310882277728286No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1183740.37636519181242445No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT1183300.37622529564907986No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC1132180.3599719050350505No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC1112590.3537433463079606No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC1107310.3520645923478261No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT999790.3178790571596329No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG935030.2972888854819227No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT923960.2937692251905044No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCAC923200.2935275863629093No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA866810.27559862124700324No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA861680.27396755916073623No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA854090.27155435034304287No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCAC853870.2714844022613706No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT694620.2208515295054203No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC681910.2168104380597178No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA667780.21231786354140333No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC657620.20908753395144758No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG640400.20361250683146348No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC598230.19020473135819238No Hit
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC581630.1849268306501938No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA563330.17910842203836405No Hit
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC540120.17172889942193953No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA498450.15848009686155992No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA482760.15349152685502393No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA477340.1517682604792798No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT475560.15120231690938596No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT474110.15074129546200055No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC466970.14847116226590962No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC465330.1479497311116254No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT453430.14416617578480714No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG451280.14348259225937357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT430570.13689793420740667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC426540.135616612529501No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCA424180.134866260380653No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGGTAGATCGGAAGAGCAC418460.133047610257174No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG403230.12820529533049818No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT393520.12511804136214477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCGCTAGATCGGAAGAGCACAC392450.12477783932855692No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG391300.12441220162890641No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT383920.12206576143462752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACAAGATCGGAAGAGCACAC382830.121719200484524No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC381280.12122638445456028No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA380810.12107694991644224No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGGAGATCGGAAGAGCACACGTCT380410.12094977158612903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG377920.12015808647992923No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC364450.1158753562066316No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGAAAAAACAC364180.11578951083367017No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC337050.1071636405801761No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGAAAGAGCAC331840.10550714282784643No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC327750.10420674440039376No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG326520.1038156710346806No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAGATCGGAAGAG314700.100057551373925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)