FastQCFastQC Report
Fri 30 Jun 2023
SRR2052988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30257426
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT461580715.255121172567687No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC22923697.5762194708829504No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC9307043.075952329851191No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT4727241.5623404317340146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC4398351.4536431486273815No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA3335451.1023574840768016No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA3138471.0372561102851248No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2925560.966889913239811No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC2489810.8228756801718692No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2385880.7885270875321648No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA1678180.5546340921398932No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT1622690.5362947925577013No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1610780.5323585687692006No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT1509190.49878333999726215No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1500280.49583860834692284No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT1448740.4788047734133102No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT1339510.44270454466285397No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTAGATCGGAAGAGCACACG1255230.41485022552810674No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1233850.4077841915568099No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT1224610.4047303957712728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT1118080.3695225099451619No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGGTAGATCGGAAGAGCAC1045960.34568703894376207No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1028090.3397810507741141No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC979370.3236792184503731No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT972020.3212500627118778No Hit
ACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTCTGA969200.32031806010200603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT879080.2905336362716379No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC728370.2407243762242036No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC723400.23908180424864955No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA723220.2390223147203599No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTCAGATCGGAAGAGCACA669080.22112918660034067No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT642690.21240736075831432No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTAGATCGGAAGAGCACACGTC628280.20764489352134582No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC613710.20282954670367534No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA598760.19788861088183776No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC597690.1975349786858935No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGAAAAAACAC597310.1974093896817264No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG579760.19160916067348227No Hit
ACGAGAGGAACCGCAGGTTCAGACATTTGGTGTAGATCGGAAGAGCACAC578780.19128527324168287No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGAGATCGGAAGAGCAC539330.17824715162486063No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAACAC534350.1766012746755127No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG523900.1731475770609172No Hit
GAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCAC510900.16885111112888454No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAAAGCAC504210.16664008366078464No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC487840.16122984156021733No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGCAGATCGGAAGAGCACA481330.15907830362040712No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC480880.1589295797996829No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTAGATCGGAAGAGCACA455080.15040274741149495No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAAAACAC444400.14687303539964042No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT436380.14422244641695564No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC428150.14150245298459954No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC421380.13926498572614868No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC403320.1332962030544171No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCCAGATCGGAAGAGCACACGT403040.13320366378818874No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC372500.12311027382170578No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC371770.12286901073475319Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC353750.1169134479581971No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGCAGATCGGAAGAGCACACG351560.1161896586973393No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG346820.11462310111904431No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA346350.11446776735073234No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA331140.10944090221025411No Hit
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGATCGGAAGAGCACACG314320.10388193628896257No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGTAGATCGGAAGAGC311040.10279790488457281No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGGAGATCGGAAGAGCACACGTCT307890.10175683813950333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC307670.10168412871603817No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGATCGGAAGAGCACACGTCTG307100.10149574520978752Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)