FastQCFastQC Report
Sat 1 Jul 2023
SRR2052989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50837419
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA572436511.260140881660416No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC41919528.245800204766493No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC38145107.503351025747393No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC26412275.195438816435587No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT18390323.6174771185767716No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA14992922.9491898477379426No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA14911092.9330934365491683No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC8313431.6352974174397013No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC4644070.9135141184095126No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA3955540.778076479453058No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3918560.7708023100071228No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT3838020.7549596489153No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC3368090.6625218325894948No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC3324110.6538707246329717No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2640090.5193202274883388No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA2542200.5000647259452727No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT2315760.4555227321827648No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA2219960.4366783451378599No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC2210850.4348863580190804No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG2198230.43240393458999166No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC2129350.41885485964580543No Hit
AACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACA1791840.35246478583029556No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG1787730.3516563262190789No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1759810.34616430861684777No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1711260.33661425651841215No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA1661520.3268301248731766No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG1414530.2782458330545853No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA1296730.2550739249764037No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC1199170.23588333624883673No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG1192270.23452606828839995No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA1166820.22951991327490487No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAAAGCA1089870.21438342493351206No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA1073170.21109844305825204No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1069600.21039620441785215No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACA1035990.20378493251201443No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCAC987390.1942250451385032No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC984560.19366836856922262No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG969200.19064697206598943No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA958580.18855795963992586No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA868690.17087610210896031No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAACA853740.1679353548613473No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA827780.1628288800420808No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC813570.1600336948655871No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG813130.15994714444492156No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTAGATCGGAAGAGCACAC782550.15393189020866696No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAAAGCAC778180.153072287167057No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTAGATCGGAAGAGCACACG753640.14824513415993837No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC737090.14498965810990525No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT728780.1433550353923357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGATCGGAAGAGCACACG712980.140247088468437No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA706400.13895276626848424No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG688520.13543567190143938No Hit
ACGAGAGGAACCGCAGGTTCAGACATTTGGTGTAGATCGGAAGAGCACAC618690.12169972673081614No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA608200.1196362860199492No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG595800.11719713780119324No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACAC585230.11511796065020533No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT574200.1129482989685216No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCACA569890.1121004982570024No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGC553450.10886665981213563No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA543380.10688583541190397No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG535600.10535546661013613No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCAGAAGAGCA526030.10347299496066076No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC508760.10007589094953857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)