FastQCFastQC Report
Fri 30 Jun 2023
SRR2052990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35106005
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC22601266.438003982509545No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC13162003.7492161241360273No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA11984703.413860392260527No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA9841512.8033693950650322No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC9710082.7659313556185046No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA8988602.5604166580617758No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA6189401.7630601944026385No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC6149841.7517914670154007No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT5929071.6889047899355112No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC5253551.4964818696972213No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT4245561.2093543540485454No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC3770291.0739729570482315No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA3367840.9593344500463667No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC3019450.8600950179321174No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2750470.7834756475423507No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA2616020.7451773564095374No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2487270.7085027191217002No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA2038570.5806898278513889No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG2027810.5776248251545568No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1685510.4801201389904661No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1575740.44885198415484756No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT1565780.4460148626994157No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1511570.43057306007903773No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG1359300.387198714294036No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1328920.3785449241518652No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1320260.37607810971370853No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1316630.3750440985808553No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1209370.34449092114012975No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACAAGATCGGAAGAGCACAC1075780.3064376023418216No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT1000970.28512785775538974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA993780.28307977509830584No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA983390.28012016747562135No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC979690.27906621673414567No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT974710.27764765600642966No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC903800.25744883247182354No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC893980.25465159023363665No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT873060.24869249577102265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC872260.24846461452962249No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT861020.24526288308795033No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTCAGATCGGAAGAGCACA839170.23903887668220866No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC819210.23335323970927482No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC756540.21550159296109028No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG705720.2010254371011455No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCTAGATCGGAAGAGCAC659470.18785105283269915No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA655370.18668316147052336No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG625280.1781119782783601No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC611770.17426363381421497No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC597030.17006492194141715No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT585830.16687458456181498No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA581720.1657038446841217No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA553430.1576453942851088No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTAGATCGGAAGAGCACAC552090.15726369320576353No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTAGATCGGAAGAGCACACG549490.15652307917121303No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGATCGGAAGAGCACACG547790.1560388315332377No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT545540.15539791554179974No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCAC541630.1542841459744565No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA538610.1534238942881709No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGTAGATCGGAAGAGC514470.1465475778289213No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC503100.1433088156855216No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAG502560.1431549958475765No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC498360.14195861933022572No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT494040.14072806062666487No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAAGATCGGAAGAGCA479570.13660625867283957No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGAAGATCGGAAGAGCA475290.13538709403134877No Hit
TGGCGCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGT473850.13497690779682847No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA461120.13135074754304857No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCAAGATCGGAAGAGCACAC454600.1294935154256373No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC446700.12724318816681077No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG443520.12633736023224515No Hit
ACGCACCGCACGTTCGTGGGGAACCTGGCGCTAGATCGGAAGAGCACACG443310.12627754140637765No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT442210.1259642046994524No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC429360.12230386225946246No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA426250.12141797393351934No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTC420390.11974874384026322No Hit
CGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTAGATCGGAAGAGCACACGT418480.11920467737642035No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAACACAC417140.11882297629707511No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC406120.11568391219678797No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT398120.11340509978278646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGCAGATCGGAAGAGCACA395570.11267872832582346No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG393880.11219732920336564No Hit
AGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACA391940.11164471719297027No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACA390750.11130574384638754No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG390300.11117756064809994No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCACAC387470.1103714307566469No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG384260.10945705727552879Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAGAGGAACCGCAGGTTCAGACATTTGGTGTAGATCGGAAGAGCACAC384170.10943142063587127No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA379710.1081609827150654No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC369820.10534380086825601No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGATAGATCGGAAGAGCACACG365080.1039936045129601No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATCAGATCGGAAGAGCACAC362610.10329002118013714No Hit
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGATCGGAAGAGCACACG361250.10290262306975687No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG359780.1024838912886841No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACA358450.10210503872485634No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG358040.10198824958863875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)