FastQCFastQC Report
Fri 30 Jun 2023
SRR2052991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35561514
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC17909505.036202901822459No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC15254134.289505221853041No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC11889333.3433137857966337No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA11295173.1762342851881953No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC9628212.707480339560346No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC7845392.206146228757302No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC7034231.978045704128345No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT6706801.885971446547523No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA5830131.6394493215333859No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT4606801.2954454076392812No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA4129991.1613650644907862No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2646150.7441049894557358No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2607140.733135265275826No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA2273000.6391741363992546No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2211150.6217817385390284No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2079150.5846629589505103No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA1873990.5269713769779318No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1854330.521442928442248No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA1718330.4831993373510476No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT1620930.4558101772607319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1445580.4065012530118937No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1410050.3965101148393176No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1369630.38514389460471227No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1176910.33095047640547587No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC1103600.31033549359006485No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1082460.3043908647983885No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT1055490.29680682324155266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1053230.2961713047425371No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC1048930.2949621323771536No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC1033800.2907075328682575No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG989930.27837116271258866No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC967780.27214251901648506No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC962360.27061839943035043No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA936960.26347584638831745No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG935690.2631187187362158No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC834530.23467223583337873No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG834410.23463849148829827No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT789890.22211933946344356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG731490.20569709152428098No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA727170.20448229510138405No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA698400.19639208836834113No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA688140.19350694686396083No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA658690.18522552217546193No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG632500.17786081886164914No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC622560.17506566227748346No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA621830.17486038417824393No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC616090.17324627967189474No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT594660.16722010204627394No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA592410.16658739557601512No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC578660.1627208560355445No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA562940.1583003468300028No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT528440.1485988476193674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC513840.14449328563457675No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGAGATCGGAAGAGCACA512040.1439871204583697No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA509130.14316882009016826No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA508540.14300291039352261No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC496290.13955817516655786No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG488510.13737041679384066No Hit
AACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACA466460.1311698933853041No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA442150.12433385147775204No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT436240.12267194248253885No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC433120.12179458951044661No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG430980.12119281535651154No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA414480.11655296790794678No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA408240.11479826196376228No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT391660.11013591828514388No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACA385260.1083362198808521No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT378670.10648309293018289No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG377070.10603316832910996No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACA370650.10422784586730474No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG358660.10085622338801435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)