FastQCFastQC Report
Sat 1 Jul 2023
SRR2052992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34060969
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC378054511.099346586410974No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC9460362.777478233223488No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA7206752.1158382194000414No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC6788361.9930026065905524No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA5309961.5589574095792753No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG4582251.3453081737046295No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC4324391.2696027526404197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC4123081.2104999126713043No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAG3996001.1731903458178186No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA3630311.0658269880695408No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC3213400.9434258902029474No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA2851510.8371781789296716No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2664720.7823382828597742No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA2633780.7732545718238375No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC2623930.7703626987241614No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAGATCGGAAGAG2233340.6556889206528446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1844660.5415759017308052No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1802400.5291687385640732No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA1742110.5114681264646347No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA1614360.47396185352213555No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1463530.42967949619988793No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1375850.4039374217451065No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA1362670.40006789002391563No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC1188210.3488479731742218No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC1160360.3406714588771682No Hit
AATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAG1002750.29439855337057497No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCTAGATCGGAAGAGCAC956980.28096088517035434No Hit
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGATCGGAAGAGCACACGT925220.2716364293687593No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC892630.2620682928897296No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG840240.24668705109358458No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA806870.23688991349600183No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG806260.23671082287764628No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC805350.23644365490600108No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG800930.23514598190086722No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG773380.22705754495710323No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA758980.2228298319992012No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG740230.21732499741859956No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG739220.21702847032919115No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACA697490.20477691048660418No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA688220.20205532026995474No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT676170.1985175465794881No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC664240.1950150038303373No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC654460.1921436821130955No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC628560.18453967061242443No Hit
AACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACA601120.17648352869819997No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGTAGATCGGAAGAGCACA600960.17643655410977885No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG586360.17215012291635037No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG569930.1673264198678552No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA543360.15952570227817064No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCAC541500.15897962268777496No Hit
TCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGATCGGAAGAGCA535940.15734725574014058No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAGATCGGAAGAGCA533670.15668080376691573No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT527660.1549163207893469No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC524540.15400031631513478No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGTAGATCGGAAGAGCACAC523690.1537507638141475No Hit
AGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAG521310.15305201681138314No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA514690.15110844321545872No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA502790.14761470820163688No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGA483380.1419161034437981No Hit
AAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAGATCGGAAGAGCACA476830.1399930812303079No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG467240.1371775418368162No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC463650.13612354950911704No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAG445190.13070385637002868No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACCAGATCGGAAGAGCA444460.13048953481035727No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA441660.12966747951298743No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC432400.12694882520811432No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGAGATCGGAAGAGCACA426130.12510800852436113No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC413190.12130893868580192No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA395740.11618577263612202No Hit
AGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAG389100.11423632721664495No Hit
TCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAA385280.11311480891809038No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA382350.11225458676762837No Hit
ATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGAGC380910.11183181547183817No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC380420.11168795579479844No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTAGATCGGAAGAGC380130.11160281435328515No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCA368520.10819422078097661No Hit
TGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGATCGGAAGAGCA365960.10744262736623847No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG362850.10652955880380266No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG360110.10572511897709075No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCACAC359750.10561942615314321No Hit
AATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCAGATCGGAAGA357530.10496765373879999No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCA355750.10444506144261485No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAGATCGGAAGAGCACAC353630.10382264814603483No Hit
TTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGATCGGAAGAGCACACGTC353430.10376392991050841No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC353000.10363768570412661No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA344620.10117739163556974No Hit
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAGATCGGAAGAGC343750.10092196731102981No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA342660.10060195292741084No Hit

[WARN]Adapter Content

Adapter graph