FastQCFastQC Report
Fri 30 Jun 2023
SRR2052994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32219386
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC21593476.702011639824546No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA13475064.1822832998741815No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC10747233.3356408467870864No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC10059053.12204894283212No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC9429042.9265113866539854No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC8805892.7331029834026013No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC6868362.1317476378972584No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA4113611.2767499666194755No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA3355431.041432012391546No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2905960.9019290435888505No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2715570.8428372905678587No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2503100.776892520546481No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC2057880.6387086333675012No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG2004350.6220944123516197No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1959430.6081524955193125No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAGATCGGAAGAG1940560.6022957731100151No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1934090.6002876653204998No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA1584550.491800185143193No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA1487370.46163821992138526No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC1450860.45030653284330124No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGA1390030.4314265951560964No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1263890.3922762525642171No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACAC1154300.3582625690011597No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA1148520.3564686179929065No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1116000.34637531578038144No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG957740.29725581983467964No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAG954030.2961043391702126No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG951040.29517632645141034No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA924200.2868459380324628No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC880900.27340682407790146No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG868630.26959855783719777No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC846350.26268346640746043No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC835890.2594369737523862No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC832180.25828549308791915No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA801950.24890294309146674No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC773850.24018148576760587No Hit
AATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAG752620.23359228509196298No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC733510.22766107336744407No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG722670.22429663929660237No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC690700.21437404176479338No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC654400.2031075328375283No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA634000.1967759410436934No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA624240.19374670889134885No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC602450.1869836998135222No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCA602400.1869681812061844No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA601330.1866360830091548No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCAAGATCGGAAGAGCACAC600080.18624811782570902No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAGATCGGAAGAGC574570.17833052436194782No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC570400.17703627250997273No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTAGATCGGAAGAGC532980.16542214677834022No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA514870.15980130720057792No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT510290.15837980276843264No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA505800.15698623182949545No Hit
AATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAG493970.1533145293333647No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC491040.15240513894336782No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACA474020.14712260500557026No Hit
TCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAA449330.13945951670214946No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG446380.13854391886921744No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGCAGATCGGA443630.13769039546563674No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA416620.1293072437817406No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAGATCGGAAGAG413650.12838543850587344No Hit
AGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGC402000.1247696029961589No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACAC399090.12386642004909715No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT398570.12370502653278372No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA395260.12267769472701932No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA392580.12184589737371158No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCTCGGGGCCACGCGCG388640.12062303111549053No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG387500.12026920686818798No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG380330.11804383857594307No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA378290.11741067939655958No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCTAGATCGGAAGAGCAC369000.11452732215319064No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACA367990.11421384628496645No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG360940.11202572265033232No Hit
AAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAG358330.11121565134729755No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA348690.10822366385256381No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGATCGGAAGAGCA341440.10597346578857834No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA339500.1053713438238705No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)