FastQCFastQC Report
Fri 30 Jun 2023
SRR2052995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29483113
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT421816014.307037387809082No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC25732438.72785380566835No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC19501186.614355817854105No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT13914834.719593212562052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC9851083.3412618267277274No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT4722081.6016219182825098No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA4248841.4411096955738696No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC3525801.1958710058873363No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC2991641.0146961075650323No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT2845620.9651694514076584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC2482360.8419599382195495No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT2247210.7622024173634582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2091490.709385742272195No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1611640.5466315582075746No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1523400.5167025612254716No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1486710.5042581494023375No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1235400.4190195248378284No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1209330.41017717498148853No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT1108410.3759474109806519No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1096660.3719620787669199No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT1057570.35870364164055535No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC978270.33180688891298554No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA910070.3086750032128561No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT890700.30210514066136773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG868130.29444991103890555No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT799200.27107042597571024No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG792400.2687640209498909No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC774960.2628487704130836No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC735030.24930542443058848No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC720940.24452641754620688No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT708670.24036471318344166No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC707220.23987290622940663No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT692340.23482594934937842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC683210.23172926142500627No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT641340.21752791165573324No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT595980.2021428334246794No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA591020.20046051446467No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT573150.19439941772770059Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG568020.1926594386420457No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC559520.1897764323597715No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA548380.18599799824394392No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA533560.18097139199649642No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC524680.1779594983745441No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG503840.17089104532482713Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC495620.16810300866126313No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC462350.15681858289523226No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT427500.14499825713790807No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC419230.14219326161386012No Hit
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC402550.1365357857564091No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC396360.13443627882849413No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG389160.13199420291880304No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC379770.12880932891991426No Hit
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC365040.12381324862133791No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC342300.11610035887323024No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA341240.11574083103097017No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC337340.11441803991322083No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA333910.11325466208402077No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGGAGATCGGAAGAGCACACGTCT333140.1129934956325677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT332340.11272215386482425No Hit
AGGAATAATGGAATAGGACCGCGGTTCTAGATCGGAAGAGCACACGTCTG331790.11253560639950062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATCAGATCGGAAGAGCACAC323460.10971026024287191No Hit
AGGATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC321460.10903190582351328No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG321300.10897763746996458No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT317700.10775659951511904No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA303100.10280461225380103No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCCAGATCGGAAGAGCACACG300220.10182778188992458No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT295610.10026417495330292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)