FastQCFastQC Report
Sun 2 Jul 2023
SRR2052996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27309344
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC289457610.599214686372548No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT273916410.030134740695345No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC12913694.728670890080699No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT6088942.22961781872168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA6074692.224399824470335No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC5750252.1055979960558555No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC5195941.9026235123040671No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3625331.327505340296713No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT3440991.2600046343112452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3313651.213375905331157No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT2578180.9440651522057798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2451580.8977073927517263No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT1914790.7011482956163283No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1580370.5786920403507313No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1407430.5153657297663393No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA1321470.4838893237420862No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC1152970.42218883031390275No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT944560.3458742912316019No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC943970.3456582479608445No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC859610.3147677219928827No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC856630.3136765203880401No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA822840.301303465949237No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT808530.2960635011957812No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT713710.26134278435981473No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG658220.2410237316575601No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG642140.23513563709183202No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC630590.23090631543547877No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA612650.22433713530431196No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT609350.22312875768821105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT588400.2154573907011461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC587040.21495939265329844No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA573970.2101734849434684No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT544500.19938230665665202No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG534910.19587068806925573No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA519730.19031215103519147No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA512520.18767202903152855No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC507910.1859839621193391No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC473930.17354133442385142No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC466410.17078769815928205No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCAC445350.16307605191834706No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG433960.15890531826762297No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT430750.15772989640468843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA424050.15527652366896838No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG423390.15503484814574822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG409910.15009880867149353No Hit
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC398050.14575597275423385No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC387200.1417829736225081No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC386080.14137285758310417No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT378920.13875104433120033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC366840.13432764990620058No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC360970.1321782024496817No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA360930.1321635554482744No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA350840.12846884934328706No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG347600.12728244222929705No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC341520.1250560980153899No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT334240.12239034375926423No Hit
ATTAGTCAGCGGAGGAAAAGAAACTAACCAGATCGGAAGAGCACACGTCT333280.12203881572548941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC330830.12114168688929328No Hit
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG318120.11648760219212881Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT317220.11615804466046492No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG314970.1153341508313052No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG308490.11296133660332522No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC308320.11289908684734426No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC304170.11137946045133855No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC292400.10706958028724528No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT281990.10325769817099964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCGACGGGCGCTGACCCCCAGATCGGAAGAGCACAC280640.10276336187350381No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA273880.10028801863567283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)