FastQCFastQC Report
Fri 30 Jun 2023
SRR2052997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36128791
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT462746312.808242047180599No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC372720310.316434336261073No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC20112205.566806816203731No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC10409612.881250579350967No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT8269482.288889213037879Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA5462721.512012953879359No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC4533401.2547887362187127No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC4177171.1561887027993825No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT3689461.021196640651496No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT2946730.8156182142934149Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC2256080.6244548841947133No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2203790.6099816625471912No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2062490.5708715799540593No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT1957110.541703706608948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1656500.4584985974205447No Hit
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1624580.4496635384228606No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT1610820.4458549415617036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1602840.44364617681228247No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1567660.43390879036057417No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1369780.3791380674764345No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1327240.3673635245641073No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC1232440.3411240636311356No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT1212930.33572393828512004No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT1211250.3352589351799788No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC1201720.3326211497085524No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1080480.2990634256208573No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC1041820.2883628184513564No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC1004790.27811337500886757No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG999220.2765716682852742No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC968520.2680742901139426No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG907000.25104631926376947No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT885330.2450483327825722No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG872790.24157741674776775No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC865270.2394959742771354No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT836480.2315272603503394No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT810430.2243169443450239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT807840.2236000645579311Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG788200.21816395682877956No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA760730.21056060248459463No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT751290.20794772789380084No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC744370.20603235796071892No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC681590.18865563478168976No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA657750.18205701928968507No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA657030.1818577322446245No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG613310.16975658000844812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC595040.1646996712400368No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT587970.16274278317256727No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC566480.15679461845263518No Hit
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA563570.1559891666455155No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT558970.15471594385762866No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC549370.1520587832568214No Hit
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC541590.14990537601991719No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA533500.1476661646386119No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC531970.14724267966785826No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC523220.14482078849524746No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG493310.13654207249835734No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCAC476050.13176471916815594No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC475700.1316678435212515No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACAC429890.11898820527927437No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT417090.115445324478198No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA416650.115323537950661No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC388920.10764821884020419No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC376960.1043378395916985No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA374440.10364033493398658No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATCAGATCGGAAGAGCACAC369290.10221487898667851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)