FastQCFastQC Report
Fri 30 Jun 2023
SRR2052998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29795235
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT679628222.809962733974075No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC11198263.7584063357781874No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA5217071.7509746105375577No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTAGATCGGAAGAGCACACG4811631.6148991608893166No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT4295601.4417070380549104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACAGATCGGAAGAGCACACGTCTGA3550791.1917308254155405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2386070.8008226818818514No Hit
AAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCTGAAC2282380.7660218152332076Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT2063580.6925872543042537No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1942170.6518391279679453No Hit
AAGAAGCCCGGCTCGCTGGCGTAGATCGGAAGAGCACACGTCTGAACTCC1775580.5959275031729067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTGAA1652000.5544510724617544Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT1462620.4908905736101763No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT1376700.46205374785599107No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT1308790.43926151278887376No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC1305250.4380734033478843No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1104700.3707639828986078No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC1073480.3602857973766611No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT1046970.35138840153467493No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGCAGATCGGAAGAGCACACG991200.3326706434770526No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAGATCGGAAGAGCACACGTCTGAAC988310.33170068972438044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT853060.28630752534759335No Hit
GAAATTCTTGGACCGGCGCAAGACGGACAGATCGGAAGAGCACACGTCTG822970.2762085950991828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT816160.2739229947338895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA790330.2652538233042968No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT773630.2596489002352222No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC765590.2569504821828054No Hit
AAGAAGCCCGGCTCGCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAG756190.25379561530560174Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC747070.2507347231864424Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC710990.23862540436415422No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGTCTG699440.23474894559482412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTAGATCGGAAGAGCACACGTC643200.21587344419334165No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCAGATCGGAAGAGCACACGTCTGA635170.21317838238228362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT616820.20701967948901895No Hit
GAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCACACGTCT587540.19719260479066536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGAGATCGGAAGAGCACACGTCTG553090.1856303533098497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC537240.1803107107562669No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC536350.18001200527534017No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTAGATCGGAAGAGCACA524540.1760482842306832No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCAGATCGGAAGAGCACAC488110.16382149696083953No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGATCGGAAGAGCACACGTCTGA473720.15899186564563092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATGTCCGGGGCTGCACGCGCGCTAGATCGGAAGAGCACACGTCTGAAC462490.15522280659978013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACTGGTGCGGACCAGGGGAATCCGACAGATCGGAAGAGCACACGTCT460370.15451128343172996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACG453820.15231294534176354No Hit
AAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCTGAAC443790.14894663525896004Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC434770.145919305553388No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGGTAGATCGGAAGAGCAC429410.14412036018511012No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGGTCAGATCGGAAGAGCACACGT416770.1398780711076788No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTAGATCGGAAGAGCACACGTC408910.1372400653997191No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA407530.13677690409221474No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCT405280.13602174978650108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC404760.13584722523584727No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCAGATCGGAAGAGCACACGT390420.1310343751274323No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC389040.13057121381992792No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGATCGGAAGAGCACA387700.13012147747785846No Hit
GATGTGTTGTTGCCATGGTAATCCTGCTCAGTAGATCGGAAGAGCACACG387370.13001072151302046No Hit
AAGAAGCCCGGCTCGCTGGCGCAGATCGGAAGAGCACACGTCTGAACTCC378110.12690284201483895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCGAAAGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA377430.1266746176024455TruSeq Adapter, Index 7 (100% over 35bp)
AACGGAATTGACGGAAGGGCACCACCAGGAGTAGATCGGAAGAGCACACG375460.12601343805477622No Hit
ATGAATGGATGAACGAGATTCCCACTGTCCCTACCTAGATCGGAAGAGCA373000.1251878026805293No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTAGATCGGAAGAGCACACG367690.12340563851904508No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT359380.12061660194994267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGGTGTCAGAAAAGTTACCACAGGGATAGATCGGAAGAGCACACGTC348100.11683076169729824No Hit
ACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTCTGA344610.1156594334631024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCAGATCGGAAGAGCACA325440.10922551877842213No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTCAGATCGGAAGAGCACA311860.10466774301327042No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGAAAAAACAC310810.10431533767060405No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC310130.10408711325821057No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTC304380.10215727447694238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)