FastQCFastQC Report
Fri 30 Jun 2023
SRR2052999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46793485
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT703634515.03701850802521No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC41296108.825181539695109No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC20130744.3020390552231795No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT13509492.8870450662095375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC5432971.1610526550864932No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT4871261.0410124400864778No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT4828751.0319278420916929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC3508510.7497860011922599No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC3300680.7053716986456555No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA2699880.5769777566257354No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT2615500.5589453318127513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCACACGTCTGA2449880.5235515157719072Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2327620.49742394694475095No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2281350.4875358182875244No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT2202100.47059969993686085No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1955770.4179577562987668No Hit
AAAAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1899490.40593044095775294No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCACACGT1810750.3869662625042781No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1696400.3625290999377371No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCTG1662230.3552268013378358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT1586760.3390984877488821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT1576410.33688664137753366No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1575920.33678192594546014No Hit
AGCATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC1526870.32629969748993903No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG1516210.32402160257993184No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCACACGT1478470.31595637726063786No Hit
AAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCACACGT1411150.301569759123519No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1352920.2891257191038453No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1308220.27957310723918083No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC1276310.2727537818565982No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC1257300.2686912505020731No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA1235850.26410727903681464No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC1150830.2459380830472447No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT1109140.23702872312246032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC1045650.22346059499522208No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC996120.21287578815726163No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT986840.21089260609676755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGAGCAGATCGGAAGAGCAC917760.1961298672240377No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT900120.1923601116693916No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA876770.18737010077364402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC859570.18369437540290062No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC846260.18084996233984282No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG840990.17972373718264412No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGAGGATCAGATCGGAAGAGCAC830240.1774264088259295No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCGCTAGATCGGAAGAGCACAC828520.17705883628885516No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC781260.16695913971784748No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCACACGT778690.16640991796187013No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACACGTC775600.1657495696249168No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCAGATCGGAAGAGCACACGTCTGA745270.15926789808453035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTAGATCGGAAGAGCACACGTCTG742320.1586374684424552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC713820.15254687698511876No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT710610.1518608840525556No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCT694420.14840100069486167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAGTCAGCGGAGGAAAAGAAACTAACCAGATCGGAAGAGCACACGTCT673940.14402432304411608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC661310.14132522935618066No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC658710.14076959645130085No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC648540.13859621697336713No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTG622440.13301851742822746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC599600.12813749606382172No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC599120.12803491768138237No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGGTCAGATCGGAAGAGCACACGT589620.12600472052893688No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT586080.12524820495844668No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGGAGATCGGAAGAGCACACGTCT585410.12510502263295842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGAGCACACGTCT572910.12243371059026699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACGGACCAGAGCGAAAGCATTTGCCAGATCGGAAGAGCACACGTCTG548590.11723640588000658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA545650.11660811328756555No Hit
AAACAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC545140.11649912375622376No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA541690.11576184163244092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA511590.10932932223364002No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGATCGGAAGAGCACACGTCTGA476600.1018517855637382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA472760.1010311585042234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)