FastQCFastQC Report
Fri 30 Jun 2023
SRR2053000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2053000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33921101
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC559813516.503400051784876No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA22762526.710430772869076No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC16697204.922363811245396No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG8952232.6391330871011527No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC8269972.438001643873529No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA7528052.2192823281296206No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT5894151.7376057457568963No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC5724111.6874776558697198No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA5274971.555070397036936No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA3977911.1726948367625214No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC3924301.1568905148450224No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC3922201.156271431166105No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3193150.9413462139687033No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACCAGATCGGAAGAGCA2997020.8835267463753609No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2045000.6028695825645518No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1687620.49751333248292856No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCCAGATCGGAAGAGCACACG1582130.46641469567865734No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC1348720.39760501877577614No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAG1298480.3827941787620632No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC1248150.3679568065906823No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1247320.3677121211366341No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC1127530.332397819280689No Hit
GTGGAGCCGGGCGTGGAATGCGAGTGCCTAGATCGGAAGAGCACACGTCT1032260.3043120563804813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACA993320.29283247616284624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC947560.2793423479974898No Hit
TTTTTGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACG908520.26783328760466824No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA886690.26139776536144865No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA875830.2581962183361914No Hit
CTGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTTAGATCGGAAGAGCAC836930.24672843018863094No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA798040.23526359005858918No Hit
AGTGACGCGCATGAATGGATGAACGAGATTCCCAGATCGGAAGAGCACAC768120.22644312164277922No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT763610.2251135657418667No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTCAGATCGGAAGAGCACACGT756680.2230705896014401No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC722630.21303258995042643No Hit
AGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGATCGGAAGAGCACACG693750.20451871535655639No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA672100.1981362574286725No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA662840.19540639320639974No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA650340.19172137130808342No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG637980.18807762165502823No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATAGATCGGAAGAGCACA635740.18741726573084994No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTAGATCGGAAGAGC635630.18738483753814478No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACAGATCGGAAGAGCACA620960.18306009583828073No Hit
AGATGTCCGGGGCTGCACGCGCGCTAGATCGGAAGAGCACACGTCTGAAC605980.17864396559533843Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC557120.16423995199919955No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCACACG551830.16268045073183207No Hit
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGATCGGAAGAGCACACGT550040.16215275559599318No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC532300.15692297251790266No Hit
AACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCACACGTCTGA523460.15431692503141334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC521920.15386293033354076No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGATCGGAAGAGCACA505520.14902818160294976No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG494450.1457647262098008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTAGATCGGAAGAGCACACGTC481890.14206201620637254No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTAGATCGGAAGAGCACACG479210.14127194751137354No Hit
AGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGAACTC479180.14126310345881757Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATAACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACA465290.13716830712540845No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCTGATCTAGATCGGAAGAGCAC461940.13618072125665967No Hit
AGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGATCGGAAGAGCAC448320.1321655213962542No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCA434320.1280382968701399No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG431850.1273101365430326No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATAGATCGGAAGAGCA430190.1268207656349362No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACAGATCGGAAGAGCAC427880.12613977358812734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT417840.12317996399939966No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACA407230.12005211741210878No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTCAGATCGGAAGAGCACA400220.11798555713153297No Hit
TTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGATCGGAAGAGCACACGT391090.11529401713700271No Hit
CGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGATCGGAAGAGCACACGT383230.1129768753673414No Hit
AATTCCGATAACGAACGAGACTCTAGATCGGAAGAGCACACGTCTGAACT383090.11293560312208026Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGAAAGAGC379150.11177408421973097No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGAGATCGGAAGAGCACA375220.1106155133349003No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCACA371770.10959844729096499No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG368540.10864623763244005No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT367200.10825120328494053No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT353450.10419767919679257No Hit
GAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCAC350110.10321304134556246No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACA346780.10223135151185099No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCACAC346510.10215175503884735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)