FastQCFastQC Report
Sat 1 Jul 2023
SRR2053001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2053001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35278187
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC19946255.653989531831667No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC19164385.432359661793278No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT13090923.710768923584423No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA11010253.120979544668778No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC10519712.9819304489768705No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA10189162.8882323232767035No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA9871432.7981681711704742No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC9174642.6006551867305423No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA6800951.9278059839072799No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC4892921.3869533601599198No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC4652461.3187922610648897No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG4320141.2245924089012852No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT3676171.0420518492064232No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2659840.7539616477456735No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA2468910.6998403857885327No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC1930170.5471284564595114No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG1915380.5429360641463803No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1783470.5055446868627348No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT1741390.49361663625174396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA1628290.4615571656219181No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG1348680.3822985574627177No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGT1298150.36797525904605016No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC1290420.36578410336109396No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1237950.3509108900635965No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT1126300.31926243828799933Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1115500.3162010564771937No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC1042880.2956160984123135No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG950580.2694526223810764No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA930180.2636700122939991No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC848670.24056508346077987No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACAC846860.24005201854619115No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA826210.23419854313941924No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG769370.2180866040536607No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT762280.21607686358712255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCAC761920.21597481752676237No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG752880.21341232756660655No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC748610.21220194790622318No Hit
AAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGATCGGAAGAGCACA666550.18894111536967587No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC664940.18848474271084284No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC659550.18695688641822777No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC654700.185582099216153No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA630870.17882721694286616No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC626670.17763667957199727No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC606490.17191643096625117No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC588970.16695018936205538No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACA578230.16390581522797643No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC574180.16275779704892432No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG572240.1622078821680944No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC563790.15981263436241777No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC560830.15897358897723401No Hit
AACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACA560190.15879217375881588No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGACAGATCGGAAGAGCACA530480.1503705391663126No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC521160.14772868004809886No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCAGATCGGAAGAGCACA506990.14371203372781033No Hit
ACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGATCGGAAGAGCACACG493480.1398824718515155No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTC487290.13812784653587784No Hit
AGCTAGGAATAATGGAATAGGACCGCGGTTCTAGATCGGAAGAGCACACG478640.13567590647444552No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC477000.13521102997724913No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT469200.13300003200277838No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCA458060.12984227335718812No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTAGATCGGAAGAGCACACG450150.12760009464205174No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTCAGATCGGAAGAGCACA445600.12631034582361048No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA442920.12555066959648464No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT437450.12400013640156735No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC436040.12360045599848993No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACAC431150.12221433034526406No Hit
CTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAG429050.12161906165982964No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC411120.11653660093133471No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG402280.11403080322693454No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT379110.10746300539764132No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG378080.10717104028049969No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAACACAC373970.10600601442472087No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCACACGT372810.10567719934133804No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG371140.10520381900577828No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG369710.1047984693771253No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCCCAGATCGGAAGAGCACAC358420.1015981915397183No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT355880.10087819989162142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)