FastQCFastQC Report
Fri 30 Jun 2023
SRR2053003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2053003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34457090
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC19191125.569570732757757No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA12703473.6867506803389376No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC9976252.8952677083294036No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6232801.8088584961759684No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC5206351.510966248165472No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC4970291.4424578511998547No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC4418861.2824240236189417No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG3869821.123083812359082No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3423310.9934994510563719No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT2837390.8234560724657829No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC2486800.7217092331360541No Hit
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2399840.6964720468269375No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA2291480.6650242373920723No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC2250840.6532298577738282No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG2067300.5999636069093472No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC1956710.567868615718855No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCAGATCGGAAGAGCACA1761920.5113374344728473No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC1558160.4522030154026356No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1550620.4500147865069279No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA1541220.44728675578814114No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1471290.4269919485365712No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA1417950.4115118252876258No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC1258470.36522817219910325No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1253240.36371034234173577No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1221120.3543886033324346No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1194430.3466427373872837No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT1186510.34434422639868895No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG1056320.3065610009434923No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT971450.28193036614525485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC852500.24740916891124584No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACG850540.2468403454847754No Hit
ATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGATCGGAAGAGCACACGT828390.24041206033359172No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG816480.2369555873696821No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC789620.2291603846987659No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG788600.22886436434417418No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG747960.21706998472593012No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC701130.20347916785776163No Hit
TCCCGTGGATCGCCCCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA691630.20072211553558353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGT645760.187409906059972No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG641430.1861532706331266No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACG640770.1859617280507437No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG628900.18251686372819065No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC619130.17968145307685587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC607930.17643103349702485No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA560940.1627937820634302No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC558890.16219883919390757No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAG558760.1621611111094988No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTAGATCGGAAGAGCACAC546590.1586291819767717No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG531340.15420338745959103No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA529480.15366358563651197No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT527060.15296126283444134No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT512010.14859351152404338No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC498710.14473363827299404No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG493200.14313454792613073No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC488220.14168927207724158No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGCAGATCGGAAGAGCACACGT465590.1351216832297794No Hit
TTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGATCGGAAGAGCACACGTC465050.13496496657146614No Hit
AAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGAGATCGGAAGAGCACA454240.13182773124486138No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA447870.12997905510883245No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC447550.12988618597798016No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACG446120.12947117704948388No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACA443500.12871081104063054No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA435350.12634555036423561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCAC429930.12477257946042455No Hit
TCCCGTGGATCGCCCCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT419810.12183559319722008Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATACATGCCGACGGGCGCTGACCCCCTAGATCGGAAGAGCACACGTCTG406370.11793508970142283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGCAGATCGGAAGAGCACACG387420.11243549585876231No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC385790.11196244372348332No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC384700.11164610824651762No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTCAGATCGGAAGAGCACAC375510.1089790228948527No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA365150.105972384783509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)