FastQCFastQC Report
Fri 30 Jun 2023
SRR2053004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2053004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40495001
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC467194511.53709071398714No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT21154745.224037406493705No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC19840994.8996146462621395No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC19771284.8824001757649045No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGATCGGAAGAGCA9833072.4282182385919686No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC8253522.0381577469278245No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA6506631.6067736360841183No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC6024881.4878083346633328No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCT5178451.2787874730513034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGAACGAGACTCTGGCATGCTAACTAGTTAGATCGGAAGAGCACACGT4302071.062370636810208No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC3475090.8581528371860023No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT3437860.848959109792342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAGATCGGAAGAGCACA2910820.7188097118456671No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT2292520.5661241988856847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCACCCACCAGATCGGAAGAGCACAC2204330.5443462021398641No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT2130590.5261365470765145No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC2063260.5095098034446277No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1922690.4747968767799265No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC1632270.40307938256378856No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA1606160.3966316731292339No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGATCGGAAGAGC1533900.37878749527626876No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG1402970.34645510936028867No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGACAGATCGGAAGAGCAC1380140.34081737644604576No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC1285910.3175478375713585No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1263500.31201382116276527No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA1248370.30827755751876634No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA1237570.3056105616592033No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1226640.3029114630717011No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGACGAGATCGGAAGAGCACACGTC1180520.2915224029751228No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACGAGATCGGAAGAGCACAC1171580.2893147230691512No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG1137770.28096554436435256No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1019110.2516631620777093No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC978390.24160759991091246No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCA939640.2320385175444248No Hit
CCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGA893650.22068156017578566No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAAAGCAC869470.21471045277909737No Hit
ACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGTAGATCGGAAGAGCA866130.21388565961512138No Hit
GATGTCGGCTCTTCCTATCATTGTGAAGCAGATCGGAAGAGCACACGTCT856220.21143844396991127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA766580.18930237833553826No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATTAGATCGGAAGAGCAC755460.18655636037643264No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAG744880.18394369221030518No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG736470.181866892656701No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA714050.17633040680749706No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACACACGT705610.17424619893206078No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTC678450.16753919823338193No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGCAGATCGGAAGAGCACACGTCT678390.1675243815897177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAACAC670830.1656574844880236No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATAGATCGGAAGAGCACA667820.16491418286420095No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCGAGATCGGAAGAGCACAC646030.1595332717734715No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCAGATCGGAAGAGCAC640380.1581380378284223No Hit
CTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAA633610.15646622653497402No Hit
AACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCA609380.1504827719352322No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGACCAGATCGGAAGAGCAC587070.14497344993274602No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTC581620.143627604799911No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGAAAGAGCAC574110.1417730549012704No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTC563920.1392566949189605No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACACGTCT555630.13720952865268482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACG533100.13164587895676308No Hit
AAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACG519190.12821088706727035No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGATCGGAAGAGCACA499020.12323002535547535No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC492970.12173601378599794No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC450170.11116680797217415No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCAGATCGGAAGAGCACACGTC448600.11077910579629323No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA435810.10762069125519963No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCA435700.10759352740848185No Hit
ACTACTCTGATCGTTTTTTCACTGACCCGGTAGATCGGAAGAGCACACGT427210.10549697232999204No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC422080.1042301492966996No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAACACAC410140.10128163720751605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)