FastQCFastQC Report
Sat 1 Jul 2023
SRR2060674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78647403
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA48894906.216975784947406No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG33364644.242306640436684No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA23393192.9744389652637353No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA19925092.5334708127616112No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC16465972.093644465285141No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC13949511.77367712955506No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA5978680.7601878475249844No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG5272840.6704404467112538No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA5216310.6632526696399625No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT4915600.6250174592541854Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC4749180.6038571928433543No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4175500.5309139069728723No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC2997140.381085692047581No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC2720240.3458779179269276No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA2675190.34014982033163893No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2543960.3234639546839201No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC2356590.2996399003791645No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC2232120.28381356724518925No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT2082080.26473601423304466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2024840.2574579608178544No Hit
TGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2016590.2564089751317027TruSeq Adapter, Index 6 (100% over 35bp)
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1837570.23364662149111268No Hit
AACGGAGAGAGGTGGTATCCCCGGTGGCGAGATCGGAAGAGCACACGTCT1726050.21946687800994524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1671020.2124698256088634TruSeq Adapter, Index 6 (100% over 49bp)
CGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1560170.19837527248038944TruSeq Adapter, Index 6 (100% over 37bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1506610.19156513025611285No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1493970.1899579570351484No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1448300.18415102657617316No Hit
TGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACACGTCT1415730.1800097582370266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1395070.1773828437793426No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1254860.1595551731059702No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG1232690.15673626248027542No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGT1214730.1544526524289683No Hit
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1192020.15156508092199816No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACG1179560.1499807946614588No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1164750.14809770641759146No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG1161650.1477035420991587No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC1142530.14527243830288966No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1027760.13067945803626854No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTC1010120.1284365359146061No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC995280.12654963317733453No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC932020.11850613808570387No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC927590.11794286455968546No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCAC894940.11379142423812774No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT881010.1120202278007832No Hit
ATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG834270.10607724707705861Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA800800.10182154393578642No Hit
CGGGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG788950.10031481904113222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)