FastQCFastQC Report
Fri 30 Jun 2023
SRR2060675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10680002
Total Bases544.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC6752956.322985707306048No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA5299554.962124538927989No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA5006774.687986013485766No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4366334.088323204433856No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3906313.657592948016302No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG3814803.571909443462651No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1950331.8261513434173513Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1814461.6989322661175532No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC1097751.0278556127611214No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC987610.9247282912493836No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC784740.734775143300535No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT740360.693220843966134No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG713950.6684923841774562No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC680510.6371815286176913No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA642380.6014792881124928No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC637570.5969755436375387No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC552410.5172377308543575No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA542790.5082302419044491No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC538260.5039886696650431No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC537490.5032676960172854No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC522250.4889980357681581No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA476880.44651677031521153No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC446450.4180242662875906No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC417320.3907489904964438No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA417260.3906928107316834No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG398330.37296809494979494No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC343450.32158233678233394No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT339150.31755612030784264No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC322780.3022284078223955No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA322140.3016291569982852No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA318350.29808046852425685No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC314070.29407297863801896No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT296460.2775842176808581No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGT291530.2729681136763832No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC279880.2620598760187498No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG279290.26150744166527307No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC264800.2479400284756501No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA258630.24216287599946146No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT257060.24069283882156575No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTC257060.24069283882156575No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG245960.2302995823409022No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA236210.22117037056734634No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT235790.2207771122140239No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG230570.21588947267987402No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT222070.2079306726721587No Hit
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT215520.20179771501915447Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA196340.18383891688409795No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC192390.18014041570404202No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC187880.1759175700528895No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA182560.17093629757747236No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACACG181750.17017787075320773No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT178000.16666663545568625No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA176880.16561794651349318No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC167500.1568351766226261No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA165510.15497188109140803No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT150910.14130147166639107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC148720.13925091025263853No Hit
TCCCGTGGATCGCCTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC148500.1390449177818506Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGT146870.1375187008391946No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA146270.13695690319159118No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC145350.13609548013193254No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC143300.13417600483595415No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG142520.13344566789406967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC137370.12862357141880684No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA128960.12074904105823202No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC128000.11985016482206651No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA123000.11516851775870456No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACG122760.11494379869966317No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT120370.11270597140337615No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA116320.10891383728205294Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC113730.10648874410323145No Hit
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA111550.10444754598360562No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC111240.10415728386567719No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT108900.10196627304002379No Hit
TGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACACGTCT108650.10173219068685567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)