FastQCFastQC Report
Fri 30 Jun 2023
SRR2060676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10272599
Total Bases523.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA7748787.543154366290361No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC6527086.353874029347393No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA4423324.3059404927613745No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG3358863.269727553854677No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA3356323.2672549566083524No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2048051.9937018859589477No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1931421.8801668399593912No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1622941.5798728247836795No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT911000.8868252328354296Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA873580.8503982293088633No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC837020.8148084043775095No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC820540.7987657261808818No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA687440.6691977366195254No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG621550.6050562277374986No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC606530.5904348062257662No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA577160.561844183735781No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC574450.5592060976973792No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC539900.5255729343664637No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC509490.4959699098543611No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC496300.4831299265161621No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT436060.42448848631198394No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA435640.4240796316492058No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC409410.39854568449522854No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC391720.3813251154844066No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT364620.3549442551003889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA351030.3417148863690678No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA319260.3107879515203504No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC300770.2927886117232844No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT291580.2838424823163057No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA274810.2675174997096645No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA262890.2559138149946279No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT252500.24579952940828315No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA240320.23394274418771724No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC221960.21606995464341594No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACG220480.21462922868886442No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG219230.21341239933535808No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC209180.20362909133316698No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG208890.20334678692315356No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC205970.2005042735533627No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC200460.1951404897631067No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT200370.19505287804965424No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG199790.19448826922962728No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG199380.19408914920167722No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT192030.18693419260305985No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA182980.17812434808367386No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGT181370.1765570718763577No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTC181210.17640131771910886No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG177750.1730331340686033No Hit
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA176860.17216675156890676No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT175610.17094992221540042No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC164410.16004713120798347No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA159230.15500459036705316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA150330.14634076537008794No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA148610.1446664081796632No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC145160.14130795916398567No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG141080.13733622815414093No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC131070.12759185869126208No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA130660.127192738663312No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC130570.1271051269498595No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACAC129420.12598564394463369No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG129260.12582988978738488No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC129120.12569360489979214No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG128720.12530421950667012No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC128650.12523607706287376No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA125640.12230595197963046No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC124550.12124487678337294No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA122590.11933688835707498No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACACG120810.11760412335768192No Hit
TCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTC120060.11687402574557812No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC114860.1118120156349917No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG114250.1112182029104806No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC114240.11120846827565253No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT112970.10997216965249008No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA111610.10864825931587517No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC111330.10837568954068974No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC110350.10742169532754076No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT108270.10539689128330619No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT107140.10429687754773646Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC103330.10058798167824909No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT102800.10007204603236242No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACGTC102740.10001363822339408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)