FastQCFastQC Report
Fri 30 Jun 2023
SRR2060677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291951
Total Bases473.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA7717618.305693820382825No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5973636.428822106358504No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG3982804.286290360334444No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3602283.8767746407616652No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3528223.7970712501604886No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA3103823.34033186356665No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA2670422.87390667471234No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1687711.816313925891344No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC1221551.3146324168089134No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1183661.2738551892923242No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC1138121.224845029854333No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1099801.1836050362297434Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA977741.0522440335727126No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG694600.7475286944582468No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA640260.6890479728100158No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT591190.6362388264854173No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTC580610.6248526278281064No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC481300.5179751808850477No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA439880.47339896648185076No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA417180.4489692207804368No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC414610.4462033861349463No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG385130.41447700273064286No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGT366780.39472872812179055No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC331220.35645904719041244No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC311200.33491351816211684No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA275880.296902125291018No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT273520.29436229269827185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA270620.2912413119698974No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA266340.2866351748949171No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT263120.28316981008617026No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC259220.2789726291066322No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC239970.25825577427173263No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG232700.2504317984457731No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC223040.2400357040195326No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC218430.23507442086166835No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC200840.21614405844370038No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA198690.21383022790369857No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC197820.21289393368518628No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA183640.19763341412368618No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC182320.1962128297921502No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA181720.19556710964145205No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT174820.1881413279084231No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAGAGCACACG169190.18208232049437195No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAGATCGGAAGAGCA163500.175958741065251No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACAC161370.1736664345302725No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC157210.16918944148543186No Hit
TGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACACGTCT156860.1688127713975246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT154810.1666065608826392No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA154670.16645589284747628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC154240.16599312673947592No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG148810.1601493593756575No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA148120.1594067812023546No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA143660.15460692808216486No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACACG142440.15329396377574525No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGT139010.14960259691425407No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG134860.14513636587192505No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG124430.13391159725228857Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC119340.12843373797386576No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCAGATCGGAAGAGC119190.1282723079361912No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA117150.12607685942381744No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC115250.12403207894660659No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA110210.11860802968074197No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA107780.11599286307041438No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA107430.11561619298250712No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACG106960.11511037886446022No Hit
ACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACACGT105310.11333464845004027No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA103090.11094548389245704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT101490.10922356349059524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCAGATCGGAAGA99930.10754469109878001No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTC98930.10646849084761639No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC95970.1032829381041721No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT95160.10241121590072956No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC94330.10151796969226376No Hit
CGGGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG94040.1012058716194263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)