FastQCFastQC Report
Fri 30 Jun 2023
SRR2060678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7836885
Total Bases399.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5231566.675560506502265No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA4977616.351515940325781No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4928606.288978337694122No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3182604.061052318618941No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA3178474.055782367611621No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG2713443.4623960923249477No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA1712792.1855494880938027No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1367531.7449917920194056No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC1152131.4701376886352167No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC996011.270925884455367No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT956271.2202169612033353Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC725700.9260056770004919No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC610420.7789064149850355No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA540190.6892917275167365No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG476780.6083794773050771No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC455060.5806643838719083No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA413940.5281945568934596No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA397880.5077017207729857No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC395230.5043202752113882No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA363590.4639470912231072No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC337250.4303367983580211No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT327470.4178573502099367No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT312070.39820668543687954No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA306990.3917245180961568No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG296570.37842841894451684No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC254240.3244146111624708No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC247730.3161077392356785No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT246660.3147424008391089No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA238840.3047639463894137No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC230950.29469617073620447No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG195310.2492189179757008No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC189330.241588335161228No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT187600.2393808254172417No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC179060.22848363858854634No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA176890.22571468127961558No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA174850.22311160620578202No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC174640.22284364259524034No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA152420.19449054056554357No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC151740.19362284887426573No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC144840.18481833024218167No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC143240.1827767027332926No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA136030.17357661877136132No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA135660.17310449240993073No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG124610.1590045024266657No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC121720.15531681273873485No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC119420.15238197319470684No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC119060.1519226070052068No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC111750.14259492132396992No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC109240.1393921181694002No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGT109120.13923899610623353No Hit
AACGGAGAGAGGTGGTATCCCCGGTGGCGAGATCGGAAGAGCACACGTCT107090.13664868120433055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA105340.13441565111648313No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA102410.13067692074083007No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA97860.12487104251242681No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT97250.12409267202466287No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA93700.11956281098931527No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC93440.11923104651912081Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCACA93210.118937562564718No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCAGATCGGAAGA91580.11685765454003728No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA90340.11527539322064827No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG89930.11475222617149544No Hit
GGTCCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC88840.11336136743106476No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA88050.1123533138485508No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG84620.1079765748763699No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC83380.10639431355698088No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT81570.10408472243755011No Hit
TGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA79680.10167304994267493TruSeq Adapter, Index 10 (100% over 35bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC79440.10136680581634155No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC78850.10061395567243873No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT78490.10015458948293869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)