FastQCFastQC Report
Fri 30 Jun 2023
SRR2060679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12238357
Total Bases624.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA7545336.165312876556878No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC7316825.9785966367871115No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2780712.2721268876206175No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2272951.8572345944802886No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2090881.708464624785827No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC2018341.649191962613936No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1753411.4327168262864043No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1458211.1915079777457056No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC1391711.1371706185724113No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1217730.9950110133247462No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA969090.7918464872368081No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA805290.6580049920099569No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA774170.6325767421231461No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC758760.6199851826515602No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA734820.6004237333491742No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC491960.4019820634420127No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC470330.38430812240564644No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC469390.3835400454489111No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC455530.372214995852793No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA435480.3558320777862584No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA407620.3330675841536572No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA402900.3292108573070715No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC395490.32315612299919017No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC381280.3115450873021599No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC334260.2731248974024863No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA332900.27201363712465654No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC320360.26176716368054964No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC310610.25380040801228465No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA296930.24262243698234984No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC280130.22889510413857023No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCAGATCGGAA277310.22659087326836438No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA275710.22528350823562346No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA272880.22297110633396297No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCA268050.2190244981413763No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG267670.21871399894610033TruSeq Adapter, Index 7 (100% over 49bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCAGATCGGAAGA262630.21459579909296647No Hit
TCGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA261060.21331294715458946No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCTAGATCGGAAGAGCAC250160.20440652286904198No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA242590.19822105205788654No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT236630.19335111731092663Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA232620.1900745336976197No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC225880.1845672584971986No Hit
TACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC214960.17564449214874184No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC212590.17370795769399439No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC206520.16874814160103355No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC203740.1664765948566462No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT203220.1660517012210054No Hit
AGGCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA197480.16136152916604737No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA188710.15419553458033622No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC186150.15210375052795078No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA184940.15111505572194045No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGC183740.15013453194738477No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA180930.14783847210863352No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA167180.1366033038585163No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC161940.1323216833762898No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA157330.12855483787570504No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC155430.12700234189932522No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCAC153220.12519654394785182No Hit
TGTTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC152940.12496775506712215No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA150530.12299853648655616No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG148730.12152775082472263No Hit
AACGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC146620.11980366318779555No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA143670.11739320890867949No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC141920.11596327840411912No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA140690.11495824153519953No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC129750.10601913312383354No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACA129220.10558606845673811No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC126410.10329000861798689No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC126210.10312658798889426No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG125430.10248924753543306No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)