FastQCFastQC Report
Sat 1 Jul 2023
SRR2060680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27383252
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC25912629.462944722562536No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA9253033.379083682244899No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC8043692.9374487734327537No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC7365112.6896403684996946No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT5858032.1392747654661326No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5728932.092129159823676No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5208531.9020859903710485No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC4229521.5445645389378881No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4221501.5416357414378685No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3018911.1024658429904526No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2263610.8266403128452384No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA2025350.7396309247710974No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1791480.6542247064008322No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1410370.5150483952746007No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1295660.47315782654302707No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1186680.4333597777210684No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1130130.4127084686654456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA944630.3449663319754717No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT930560.33982815481521333Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG918290.3353473137522161No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC889330.3247715063207248No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC858270.31342880677576207No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA829330.3028603030786847No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC811830.2964695354664231No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC747470.2729661181221281No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC743120.2713775558870802No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC704960.2574420306251427No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT679950.248308710740419No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA675630.24673110410699212No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA674170.24619793149476912No Hit
GCCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT657280.24002992778213486No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG617370.2254553257589712TruSeq Adapter, Index 9 (100% over 49bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA587060.21438651625453395No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC580090.21184116481125032No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA568860.20774011793778183No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT567000.20706087063727857No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC551230.20130187605182903No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA543880.19861775365467912No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG523820.19129210803742375No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC517110.18884170514152226No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC501010.18296219893824153No Hit
TATATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC483280.1764874383802187No Hit
GATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC472470.17253976992944448No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG466970.1705312429655908No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA447620.1634648799200329No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG430660.15727131313694956No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA414110.1512274729093535No Hit
GGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA412290.1505628330776783No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG412180.15052266253840121No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC398400.14549038952714602No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC387360.14145872813061064No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG379730.13867235345166454No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG368360.13452018043729796No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC365630.13352322068978512Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA357560.13057616385373075No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC350990.12817688709872735No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG349320.12756702527515723No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC346020.12636190909684503No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC338500.12361570495717603No Hit
GCGCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG332300.12135154728883187No Hit
GCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC321900.11755360539354492No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC320210.1169364398355608No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC319220.11657490498206714No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC311870.11389078258491722No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG309480.11301798632244264No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT309440.1130033788536146TruSeq Adapter, Index 9 (100% over 36bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA309360.11297416391595856No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC308890.11280252615722924No Hit
TCGCTGCGATCTATTGAACGTCAGCCCTCGACACAAGATCGGAAGAGCAC308530.11267105893777701No Hit
TACTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG306320.11186399628502852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG287350.10493640419333686No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG285800.10437036477625083No Hit
GACAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG278470.10169354611351492No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC276860.10110559549318687No Hit
TGGGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA275740.10069658636600212No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)