FastQCFastQC Report
Fri 30 Jun 2023
SRR2060681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8062047
Total Bases411.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3767334.6729199172369No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT3630194.502814235640154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA3144163.899952456243433No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT2750533.4117017675535752No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1314121.6300078627673593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1293771.604766134456919No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC914051.1337691283615687No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA847951.0517800255939962No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA666540.8267627315990591No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT641720.7959765057187089No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC620280.7693827634594539No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA614730.7624986557384248No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC562460.6976640051837951No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC549720.6818615669196669No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG504910.6262801494459161No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA427200.5298902375538124No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC422080.5235394931336917No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA420490.5215672893000997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC400330.4965612331458747No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG381780.47355218842063307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT330470.40990830244477616Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA316880.3930515413765263No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC310800.38551003237763315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT298200.3698812472812426No Hit
GCCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT295650.36671827886887787No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG293220.36370415602885964No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC289260.35879225214142263No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC281960.34973747982367254No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG268570.33312879470933376TruSeq Adapter, Index 10 (100% over 36bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTAGATCGGAAGAGCACACGT268080.3325210086222519No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG263850.32727420219703507No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC244940.30381862075475374No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT240080.2977903750747174No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC238950.29638874593512043No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC228400.2833027393663172No Hit
ACTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT222500.27598449872594394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA219350.2720773024518463No Hit
TTTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT218800.27139509357859115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG211240.26201782252075684TruSeq Adapter, Index 10 (100% over 49bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT208640.25879283511991436Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT198410.2461037500773687No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC184550.22891208647133912No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC180340.22369008764151335No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT179340.22244970787195859No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA179050.22208999773878768No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC178300.22115971291162156No Hit
ATGGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT172260.21366781910351057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC172110.21348176213807735No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT171710.21298561023025542No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC166180.20612631010461735No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC162960.20213228724665086No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG158270.19631490612743882No Hit
AAAAAACCGATCTCAGTTCGGATTGAAGTCTGAGATCGGAAGAGCACACG157720.1956326972541837No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA151590.18802916926681276No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC149540.1854863907392254No Hit
GGTAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG149310.1852011033922278TruSeq Adapter, Index 10 (100% over 36bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC141200.17514162346113835No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC140920.174794317125663TruSeq Adapter, Index 10 (100% over 37bp)
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA137190.17016770058522357No Hit
TCGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA136780.1696591448797061No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT130160.1614478308052533No Hit
GACGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC126680.15713130920720259No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA113240.14046060510438602No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG112020.13894734178552917No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG107540.13339044041792364No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG106120.1316291011451558Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC104220.1292723795830017No Hit
ATTCTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT102120.1266675820669366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA101950.1264567175061123No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC100090.12414961113474035No Hit
TACCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA98590.12228904148040814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCT92310.11449945652760396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTAGATCGGAAGAGCACACG91060.11294898181566046No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA90530.1122915805377964No Hit
CTCTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTC89190.11062947164659298No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAAGATCGGAAGAGCACACGTCT88330.10956274504477585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGAGTGGACGTACCGCTGGTGTTCCAGTAGATCGGAAGAGCACACGT86460.10724323487570836No Hit
TCGCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT86020.10669746777710425Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTCTTAGCTCAGCTGGCAGAGCAACTGAGATCGGAAGAGCACACGTC85740.1063501614416289No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA84450.10475007153890321No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG83080.10305075125461313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG82820.10272825251452888Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)