FastQCFastQC Report
Fri 30 Jun 2023
SRR2060682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18856341
Total Bases961.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA9734345.162369518031096No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8518424.5175360373468No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC5037922.6717378520042674No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC4275992.26766688192582No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3517131.8652240113816356No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC2755681.4614075975821608No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2655741.4084068589977239No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1830540.97078218939719No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1545430.8195810629432296No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1304230.6916665327594574Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1206810.6400022146396271No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1156830.6134965420915967Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA1154140.6120699662781873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1151740.6107971848833238No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1093780.5800595141973727No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA1013730.5376069514228662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA934360.495515010043571No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC910140.48267052446707454No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA839500.44520832541159494No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC829700.4400111347159027No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA819150.4344161998343156No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA757320.40162616914914717No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG710620.37685996450743015Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC699620.3710263831143062No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC640040.3394295849868222No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG627480.33276869568703704No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA616640.327019966386904No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC613210.32520094964341173No Hit
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC577670.30635317848780946No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGA574520.30468265290705127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC560020.2969929319797515No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA507960.26938418222283955No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC496660.2633915031553577No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC480390.25476310594934615No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG458000.242889116186433No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT457220.24247546223310237No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA453730.24062462595473852No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC448010.23759116363031405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC439690.2331788547947876No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACAC438570.23258489014385134No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT403770.214129559918332No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC370320.19639016922742328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC364160.19312336364727387No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC358870.19031794132276245No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT343290.18205546876777418TruSeq Adapter, Index 11 (100% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG334640.177468152490454No Hit
TCCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC332610.17639159156063203No Hit
TACCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT332470.17631734597926502Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA328600.17426498598004778No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT319070.16921098319127767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG318250.16877611621469935No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG312680.16582220272745385No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC310090.16444865947216378No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA291180.15442020273180251No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC281870.1494828715708949No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA281250.1491540697105552No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC270860.14364398692195904No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG264000.14000595343497446No Hit
AGGCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA262840.1393907757607905No Hit
TCGTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC252850.13409282320467159No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC249250.13218365111237645No Hit
AAAAAACCGATCTCAGTTCGGATTGAAGTCTGAGATCGGAAGAGCACACG245480.13018432367127855No Hit
TACCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA241770.1282168157650522Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG240760.12768118692804717No Hit
TCGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA237950.1261909720448946No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG229470.12169381111637725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG221250.11733453483897009Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG209290.11099184088790078No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG205330.10889175158637616TruSeq Adapter, Index 11 (100% over 49bp)
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA200660.10641513112220446No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC196890.10441580368110653Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAGA196840.10438928740204687No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA195360.1036044055418811No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG195310.10357788926282147Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC195200.10351955344889022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)