FastQCFastQC Report
Fri 30 Jun 2023
SRR2060683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8565355
Total Bases436.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4668485.4504220782442765No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2253852.6313562018153362No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2162252.5244137575150125No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1214341.4177345830966726No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1103761.2886331039402337No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC945871.1042974867941842No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC944801.103048268285436No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT802530.9369489063792452Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC795150.9283328011506821No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA654220.7637978811152603No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC612540.7151367339707462No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT559110.6527575331086686No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC522020.6094551831185047No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG513160.5991111868684953TruSeq Adapter, Index 12 (100% over 49bp)
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA510030.5954569308569231No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT508270.5934021415341221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC508240.5933671167161197No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA458880.5357396161630195No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA456680.5331711295095183No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC447580.5225469347154904No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT436110.5091557792992818TruSeq Adapter, Index 12 (100% over 36bp)
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG407220.47542687956307705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC323020.37712389036998467No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA318930.3723488401823392No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA317010.37010725183019266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA315980.36890473307878074No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC298110.3480416164887503No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA297120.34688579749467474No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC263490.3076229765141083No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG242480.2830939289731716No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC241370.2817980107070869Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC240300.2805487921983385No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA237920.2777701566368236No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC237770.2775950325468121Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT236110.2756569926173521TruSeq Adapter, Index 12 (100% over 36bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA229450.26788148302084386No Hit
AAAAAACCGATCTCAGTTCGGATTGAAGTCTGAGATCGGAAGAGCACACG228940.2672860611148049No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA224880.2625460357451617No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA216780.2530893348845436No Hit
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT207990.24282706320987282Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC205750.2402118767990352No Hit
TACCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT202950.23694289378548816Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC194280.2268207213828265No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACA189150.22083147750443502No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC177250.2069382996968602No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC165480.19319689610062862No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG158690.18526961229277714TruSeq Adapter, Index 12 (100% over 37bp)
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG155650.1817204307352118No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA155360.18138185749452299No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC150780.1760347352795068No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA147580.17229875469259592No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC147020.17164495808988653No Hit
TCGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA146750.17132973472786592No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACA145320.16966021840309012No Hit
TACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC141280.16494354291211516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA139730.1631339273153302No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGC137280.16027356717847654No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC137100.16006341827046283No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA134920.15751828149562977No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC133900.15632743768355192No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA129710.15143563810256552No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG128520.15004632032180804No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG120730.14095154258054687No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC119710.13976069876846903No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGTAGATCGGAAGAGCACAC117380.13704043790362455No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG117070.13667851478426754Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT116960.1365500904515925TruSeq Adapter, Index 12 (100% over 36bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT112910.1318217400212834No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA111360.13001212442449847No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT110420.1289146801270934No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC109350.12766546161834508No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC107190.12514367472218024No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC105550.1232289846713884No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA104910.12248178855400622No Hit
ATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTGAACTCCAG103450.12077724741122813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA102810.12003005129384596No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC101770.11881585760309993No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG101270.11823211063639512No Hit
GGGTCAGTACTCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC98200.1146479042608275Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC97340.11364385947809519No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACAC96670.11286163854271071No Hit
GGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA95510.11150734557995552No Hit
TCTCGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC93990.10973275480117287No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC91150.10641707203028947No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC88060.10280951577605366No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA86690.10121004908728243No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCTAGATCGGAAGAGCAC86460.10094152548259822No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACAC85950.10034610357655929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)