FastQCFastQC Report
Fri 30 Jun 2023
SRR2060684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4063729
Total Bases207.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1914684.711633083800618TruSeq Adapter, Index 1 (100% over 36bp)
GCCCGGATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC644651.5863508614870725TruSeq Adapter, Index 1 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG457651.1261823807640716TruSeq Adapter, Index 1 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC365250.8988050138185887No Hit
TGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT355630.8751321753985072TruSeq Adapter, Index 1 (100% over 35bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA324090.7975187321792374No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA284550.7002189368434756TruSeq Adapter, Index 1 (100% over 40bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA183410.45133423021072516No Hit
GGGGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT164360.40445610423332856TruSeq Adapter, Index 1 (100% over 41bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA151330.37239195822359217Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC149010.36668291610980946TruSeq Adapter, Index 1 (100% over 50bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC146010.359300534066125No Hit
TATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCG138160.3399833010518172TruSeq Adapter, Index 1 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC133340.32812227390163073No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC128020.3150308497441635TruSeq Adapter, Index 1 (100% over 36bp)
GGCTCCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT123340.3035143337560157TruSeq Adapter, Index 1 (100% over 41bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG112960.2779712918848673No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT99570.2450212600298888TruSeq Adapter, Index 1 (100% over 48bp)
GGGGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT95090.23399690284465327TruSeq Adapter, Index 1 (100% over 41bp)
AGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC89910.2212499898492247TruSeq Adapter, Index 1 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA86970.2140152554464139Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCAGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC86030.21170210907272605TruSeq Adapter, Index 1 (100% over 36bp)
TACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA80570.19826617375322025Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACACGTCTGAACTCC77860.19159742197375856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG75770.18645436248332503No Hit
ATGCGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA72550.178530605756437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT70260.17289538746309116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC63660.15665414696698526No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA63230.15559600554072378No Hit
GCCGGAATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC61070.15028069046927095TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG57890.1424553655029654No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT57520.14154487171757762No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA56060.13795211245631783No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT54850.1349745516986984No Hit
GGGGCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC54600.13435935319505804TruSeq Adapter, Index 1 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC53240.13101267333525438No Hit
TCCCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC51880.12766599347545077TruSeq Adapter, Index 1 (100% over 36bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA50910.1252790232813261No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT49820.12259675780545405Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA49780.1224983260448716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGGCTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC47980.11806889681866088TruSeq Adapter, Index 1 (100% over 36bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC46780.11511594400118708No Hit
GCCGTGCTGCTGCCCAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT45430.11179387208152906Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT45120.11103102593701498TruSeq Adapter, Index 1 (100% over 48bp)
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC43480.10699532375313414Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC42850.10544502352396037TruSeq Adapter, Index 1 (100% over 36bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT42730.105149728242213No Hit
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC41820.10291040568896202TruSeq Adapter, Index 1 (100% over 36bp)
GTTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT41200.10138471339993389TruSeq Adapter, Index 1 (100% over 43bp)
TATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGTCTGAACTCCA41090.10111402605833214Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGCATTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA40640.10000666875177947TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)